ADHD大鼠前额叶皮层、纹状体中NGFI-B基因mRNA表达的变化  

Changes of the NGFI-B gene mrNA expression in prefrontal cortex and striatum of SHR/WKY Rat -an animal model of attention deficit hyperactivity disorder.

作　　者：池霞[1] 郭锡熔[1] 陈荣华[2] 费莉[2] 郭梅[2] 潘晓琴[2] 

机构地区：[1]南京医科大学附属南京妇幼保健院儿保所,江苏南京210011 [2]南京医科大学儿科研究所,江苏南京210029

出　　处：《中国儿童保健杂志》2005年第3期238-240,共3页Chinese Journal of Child Health Care

基　　金：江苏省教育厅自然科学基金资助项目(02KJB320011);南京医科大学科研基金资助项目(NY01 01);江苏省卫生厅135重点学科资助项目

摘　　要：【目的】　探讨注意缺陷多动障碍(attention deficit hyperactivity disorder,ADHD)大鼠前额叶皮层、纹状体中NGFI B基因mRNA表达水平的变化,分析NGFI B基因与ADHD病理生理学之间的关系。　【方法】　以幼年SHR大鼠为ADHD实验组、WKY大鼠为对照组,应用RT PCR技术,检测两组大鼠前额叶皮层及纹状体中NG FI B基因的mRNA表达丰度。　【结果】　ADHD大鼠前额叶皮层、纹状体中NGFI B基因mRNA的表达丰度分别为(123.13±14.36)%、(79.01±8.67)%,均显著高于对照组[分别为(45.14±7.79)%、(36.66±6.83)%],差异有非常显著性(P均<0.01)。　【结论】　NGFI B基因在大鼠前额叶皮层、纹状体中表达显著上调可能与ADHD的病理生理学相关。【Objective】To investigate the changes of the nerve growth factor inducilble-B(NGFI-B) gene mRNA expression in prefrontal cortex and striatum of SHR/WKY rats, an animal model of attention deficit hyperactivity disorder(ADHD), and to analyze the relationship between the NGFI-B and the pathophysiology of ADHD. Six weeks old SHR rats were used for ADHD model, and same old WKY rats were used for the control group. Each group had 8 rats. The expressive levels of NGFI-B in the PFC and striatum of SHR /WKY rats were verified by using RT-PCR technique. The levels of NGFI-B gene mRNA expression (123.13±14.36)% in the prefrontal cortex of SHR were significantly higher than those (45.14±7.79)% of WKY rats ( P <0.01). The levels of NGFI-B gene mRNA expression (79.01±8.67)% in the striatum of SHR rat were significantly higher than those (36.66±6.83)% of WKY rats ( P <0.01). [Conclusion] The higher expression of NGFI-B in the SHR rats may be one of the important pathophysiological bases for ADHD.

关 键 词：注意缺陷多动症 NGFI-B基因 RT PCR 多巴胺 

分 类 号：R749.94[医药卫生—神经病学与精神病学]