丹贝异黄酮生物活性增强的机理研究  

Machanism of Activity Enchencement of Tempe Isoflavone

作　　者：徐德平[1] 江汉湖[2] 

机构地区：[1]江南大学食品学院,江苏无锡214036 [2]南京农业大学食品科技学院,江苏南京210095

出　　处：《食品与生物技术学报》2005年第3期8-11,共4页Journal of Food Science and Biotechnology

基　　金：国家自然科学基金项目(39470610)资助课题.

摘　　要：以少孢根霉RT-3孢子接种在含高浓度的大豆异黄酮提取物制成的发酵基质中,发酵36h后大豆异黄酮被部分水解成相应的苷元.用从丹贝中分离的β-葡萄糖苷酶与标准品染料木素糖苷和大豆苷元的糖苷作用10min,染料木素糖苷和大豆苷元的糖苷被水解成相应的苷元.结果表明:丹贝异黄酮生物活性增强是发酵剂RT-3孢子分泌的β-葡萄糖苷酶将异黄酮由糖苷水解成苷元,而苷元比糖苷有更强的活性.The culture medium containing extraction of soybean isoflavone was inoculated with spore of RT-3. After 36 h fermentation, the some of soybean isoflavones were hydrolysized to isoflavone aglycones. Genistin and daidzin were reacted with α-Glucosidases, respectively, for 10 minutes, genistin and daidzin were then hydrolyzied to genistein and daidzein. The reason of activity enhencement of tempe isoflavones was because that, soybean isoflavones were hydrolyzed to isoflavones aglycones, and isoflavone aglycone have a stronger activity than soybean isoflavones.

关 键 词：丹贝 异黄酮 Β-葡萄糖苷酶 水解 生物活性 

分 类 号：O623.52[理学—有机化学]