仿生鲶鱼抗菌肽编码序列的设计及克隆  

Design and Cloning of an Emulated Sequence of Catfish Parasilurus asotus Antimicrobial Peptide

作　　者：谢慧[1] 江龙法[1] 邬敏辰[2] 王武[1] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214036 [2]江南大学医学系,江苏无锡214063

出　　处：《食品与生物技术学报》2005年第3期34-38,共5页Journal of Food Science and Biotechnology

摘　　要：人工合成的仿生鲶鱼抗菌肽DNA序列,经聚合酶链式反应(PCR)扩增后得到带有不同限制性酶切位点的序列XH1、XH2、XH3.将此基因克隆至载体pGEX-5X-3,成功构建了三串联的重组质粒.测序验证后转化大肠杆菌BL21(DE3),37℃IPTG诱导,获得高效表达,SDS-PAGE扫描分析表明,融合蛋白可达细菌全蛋白总量的30%,融合蛋白以包含体的形式存在于大肠杆菌细胞中.Sequences XH1、XH2、XH3 coding for antimicrobial peptide with different restriction sites were obtained by PCR amplification,and then cloned into the fusion vector pGEX-5X-3 . The recombinant with three sequence segments was constructed and transformed into E. coli BL21 (DE3) after the expected sequences were checked. Induced by IPTG in 37℃,the cloned protein expressed in a high level and reached about 30% of the total bacterial protein from desitometric scan analysis of SDS-PAGE. The fusion protein existed in E. coli cells was in the form of inclusion body.

关 键 词：抗菌肽 融合蛋白 大肠杆菌 表达 编码序列 克隆 仿生鲶鱼 

分 类 号：Q811[生物学—生物工程]