苏皖产大戟属药用植物rDNA的ITS序列分析  

ITS sequence analysis on medicinal plants of Euphorbia L. in Anhui and Jiangsu Provinces

作　　者：蒋继宏[1] 孟娜 曹小迎[1] 周守标[2] 戴传超[3] 

机构地区：[1]徐州师范大学江苏省药用植物生物技术重点实验室,江苏徐州221116 [2]安徽师范大学生命科学学院,安徽芜湖241000 [3]南京师范大学生命科学学院,江苏南京210097

出　　处：《中草药》2005年第6期900-902,共3页Chinese Traditional and Herbal Drugs

基　　金：江苏省药用植物生物技术重点实验室开放项目(KJS03079)

摘　　要：目的研究苏皖产大戟属内6种药用植物的ITS长度的变异,为探讨大戟属植物的系统演化关系和大戟属植物鉴定提供DNA分子证据.方法利用PCR技术对大戟属植物的rDNA ITS区碱基序列进行测定.结果这6种大戟属植物的ITS1的长度范围为255～262 bp,ITS2的长度范围为214～236 bp.运用Mega2软件进行的系统分析得到大戟属内6种植物的系统进化树.这一分析结果与来自形态学的研究结果相吻合.结论此法可用于大戟属植物种间及真伪品鉴别.Objective To study the correlation and variation between plants of internal transcribed spacers (ITS) sequence of the six medicinal plants of Euphorbia L. in Anhui and Jiangsu Provinces, in (order) to provide their DNA molecular marker to identify and explore the phylogenetic relationship of the plants of [WTBX]Euphorbia L. Methods To determine rDNA ITS sequence for the plants of Euphorbia L. by PCR technology. Results The sequences of ITS1 in the six species ranged from 255 to 262 bp in length and those of ITS2 from 214 to 236 bp. The dendrogram was obtained with Mega2 analysis. The analysis result was consistent with those from morphology. Conclusion The method can be used to identify the plants of [WTBX](Euphorbia) L. among different species and to differentiate their fakes.

关 键 词：药用植物 ITS序列分析 rDNAITS区 大戟属植物 DNA分子 PCR技术 系统进化树 植物鉴定 碱基序列 ITS1 ITS2 系统分析 分析结果 伪品鉴别 长度 形态学 

分 类 号：R282.71[医药卫生—中药学] R282.5[医药卫生—中医学]