非促有丝分裂型人酸性成纤维细胞生长因子对细胞增殖活性的影响  

The mitogenic activity of the non-mitogenetic human acidic fibroblast growth factor on nasopharyngeal and angioendothelial cells

作　　者：郭钦丽[1] 汪小凤[2] 孟娟[2] 郑青[2] 

机构地区：[1]暨南大学药学院,广州510630 [2]暨南大学药学院医药生物技术研究开发中心,广州510630

出　　处：《广东医学》2005年第6期758-760,共3页Guangdong Medical Journal

基　　金：国家863计划资助项目(编号:2001AA215131;2002AA2Z3318);广东省自然科学基金资助项目(编号:010424)

摘　　要：目的研究观察人酸性成纤维细胞生长因子(haFGF)和N端缺失27个氨基酸残基的非促分裂人酸性成纤维细胞生长因子(non-mitogenetichumanacidicfibroblastgrowthfactor,nm-haFGF)对鼻咽癌细胞及血管内皮细胞增殖活性的影响。方法用不同浓度(0.01～100ng/ml)的人酸性成纤维细胞生长因子(haFGF)及nm-haFGF分别刺激鼻咽癌细胞及血管内皮细胞,作用48h后采用MTT法测定haFGF和nm-haFGF对两种细胞的增殖活性。结果在各浓度(>0.01ng/ml)下,nm-haFGF组对鼻咽癌细胞(除浓度为50ng/ml外)和血管内皮细胞的促增殖作用都显著低于haFGF组。结论nm-haFGF对鼻咽癌细胞及血管内皮细胞的促分裂活性下降。Objective To study the mitogenesis of non-mitogenetic human acidic fibroblast growth factor (nm-haFGF) on nasopharyngeal and angioendothelial cells. Methods The mitogenic effects of human acidic fibroblast growth factor (haFGF) and nm-haFGF on CNE2 cells and ECV304 cells were measured by MTT method. Results At different concentration(>0.01 (ng/ml)), the mitogenic effects of nm-haFGF on nasopharyngeal cells(except for the concentration of 50 (ng/ml)) and angioendothelial cells was significantly lower than that of haFGF. Conclusion The mitogenic effect of nm-haFGF on CNE2 cells and ECV304cells is lower than that of haFGF.

关 键 词：人酸性成纤维细胞生长因子 有丝分裂 血管内皮细胞 鼻咽癌细胞 内皮细胞增殖活性 factor 氨基酸残基 human 促增殖作用 研究观察 不同浓度 MTT法 种细胞 

分 类 号：R329.26[医药卫生—人体解剖和组织胚胎学] R329.25[医药卫生—基础医学]