幽门螺杆菌OMP25基因的克隆、测序及生物信息学分析  

Cloning,sequencing and analysis of the biological information of outer membrane protein 25 of Helicobacter pylori

作　　者：李良仁[1] 王继德[1] 白杨[1] 王群英[1] 

机构地区：[1]南方医科大学南方医院消化病研究所,广州510515

出　　处：《广东医学》2005年第6期763-765,共3页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目(编号:302050302)

摘　　要：目的克隆幽门螺杆菌(helicobacter pylori,Hp)外膜蛋白25(OMP25)基因,并对其进行测序及生物信息学分析。方法利用PCR技术扩增OMP25基因,并将其定向插入pET-22b(+)载体中,以DNA自动分析仪进行序列测定,并以生物信息学软件分析其生物学特性。结果成功克隆了幽门螺杆菌OMP25基因,经测序表明其序列与Gene Bank公布的一致。DNAstar软件预测其蛋白质的相对分子量(Mr)约为72.4 kD,并显示出良好的抗原性。结论本研究获得了序列正确的OMP25基因,为其重组表达及其相关研究奠定了良好基础。Objective To clone the outer membrane protein 25(OMP25) gene of Helicobacter pylori(Hp) and to perform sequencing and analysis of biological information. Methods The OMP25 DNA was amplified by PCR. The PCR products were inserted directionally into vector pET-22b(+) to construct recombinant clones of OMP25 and was sequenced. The biological property at the amino acid level was analyzed by DNAstar 5.0. Results The recombinant plasmid was constructed. DNA sequence analysis showed the sequence of OMP25 was the same as what was published by GeneBank. DNAstar 5.0 software predicted its relative molecular mass(Mr) was 72.4 kD and possessed good antigencity. Conclusion A confirmed OMP25 gene has been obtained, providing a good foundation for recombination, expression and related study.

关 键 词：生物信息学分析 幽门螺杆菌 基因 克隆 测序 PCR技术 自动分析仪 生物学特性 相对分子量 外膜蛋白 序列测定 软件分析 相关研究 DNA 蛋白质 抗原性 表达及 

分 类 号：R394[医药卫生—医学遗传学] R573.1[医药卫生—基础医学]