溶血磷脂酸通过G_i通路促进卵巢癌细胞转移的初步研究  

Lysophosphatidic acid stimulates ovarian cancer cell migration via Gi-dependent signaling pathway

作　　者：王进胜 李国红 滑玮[2] 谭小燕[2] 

机构地区：[1]陕西省铜川市矿务局中心医院妇产科,陕西铜川727000 [2]第四军医大学西京医院妇产科,陕西西安710033

出　　处：《徐州医学院学报》2005年第3期206-208,共3页Acta Academiae Medicinae Xuzhou

摘　　要：目的研究溶血磷脂酸(LPA)对卵巢癌细胞转移的影响。方法建立测定卵巢癌细胞转移的实验模型,用Transwell板测量0.05、0.5、5、50、100μmolL5种浓度的LPA的趋化作用,用化学抑制剂初步确定介导LPA促进卵巢癌细胞转移的通道。结果细胞发生最大趋化作用时的LPA浓度是50μmolL,Gi通道抑制剂百日咳毒素完全阻断了LPA对卵巢癌细胞迁移的促进作用。结论Gi依赖型信号通路对LPA促进卵巢癌细胞的转移起重要作用。Objective To evaluate the effect of lysophosphatidic acid (LPA) on ovarian cancer cell migration. Methods Experimental model for evaluating ovarian cancer cell migration was established. Varied concentrations of LPA (0.05, 0.5, 5, 50, and 100 μmol/L) were added to Transwell to asasess the chemotaxis. The chemical inhibitor on cell migration was tested. Results Maximum chemotaxis was detected at the LPA concentration of 50 μmol/L. Gi inhibitor pertussis toxin (PTX) completely abrogated the LPA-stimulated cell migration. Conclusion Gi-dependent signaling pathway is essential for LPA-stimulated ovarian cancer cell migration.

关 键 词：溶血磷脂酸 Gi通路 卵巢癌 细胞转移 肿瘤 LPA 

分 类 号：R737.31[医药卫生—肿瘤]