不同氮素浓度对小麦醇溶蛋白差异表达的研究  

Alteration of Gladins Expression in Wheat Seeds with Different Nitrogen Density

作　　者：周洪华[1] 魏凌基[1] 孙杰[1] 王金玲 

机构地区：[1]石河子大学生物化工实验室,新疆石河子832003

出　　处：《石河子大学学报（自然科学版）》2005年第2期214-215,共2页Journal of Shihezi University(Natural Science)

摘　　要：利用穗离体培养技术和A PAGE技术研究了不同氮素浓度影响下小麦籽粒醇溶蛋白的表达差异,研究发现低氮条件下(0%～0.09%)小麦籽粒醇溶蛋白的表达不受影响,但在高氮(0.12%～0.2%)胁迫下,籽粒醇溶蛋白出现了差异表达,共产生了4条特异蛋白谱带,其中2条在ω区,另2条在γ区,且γ区的蛋白特异带表达比ω区强。Vitro culture and A-PAGE technology were used to analyze the difference of the gladins expression in wheat seeds.The results showed in low nitrogen,the gladins expression were little influenced,but until high nitrogen stress,the gladins appear different expression,prouduce 4 peculiar tables altogether.Two of them are in ω district among them,the another two are in γ district,and in γ distict their expression is stronger than ω district.

关 键 词：小麦 氮素 醇溶蛋白 差异表达 

分 类 号：S512.1[农业科学—作物学]