流动注射分析测定十七合氨基酸输液中乙酰半胱氨酸含量  

Flow injection analysis for determination of N-Acetyl-l-Cysteine in in-jection of 17 amino acid

作　　者：张少辉[1] 

机构地区：[1]沈阳第一制药厂药物研究所

出　　处：《中国药学杂志》1994年第4期219-221,共3页Chinese Pharmaceutical Journal

摘　　要：本文采用流动注射分析，用亚硝基铁氰化钠的碱性缓冲液（ｐＨ＝１２）为显色剂，测定乙酰半胱氨酸的含量。乙酰半胱氨酸在浓度４０～２００×１０￣（－６）ｇ／ｍｌ内线性方程为Ｙ＝５．１７×１０￣４ｘ－１．８×１０￣６（ｒ＝０．９９９１）；样品测定平均回收率为９９．１４％（ＲＳＤ＝１．３％，ｎ＝５）。本法具有分析速度快（１８０次／ｈ）、选择性好等优点，适用于大批量样品的分析和生产监测。A new method was introduced for determination of N-Acetyl-L-cysteine with sodi-um nitroproussde in the buffer solution(pH=12)by flow injection analysis. The calibration graphfor N-acetyl-L-cysteine is linear from 40× 10￣(-6) to 200 × 10￣(-6)g/ml(Y=5. 17 × 10￣4X-1.8 × 10￣6,r=0. 9991,n=5). The rate of recovery was 99. 14%(RSD=1.3%,n=5). The method with higherspeed(180times/h)and bettter selection is suitable for determination of large samples and produc-tion control.

关 键 词：流动注射分析 乙酰半胱氨酸 氨基酸 

分 类 号：R927.2[医药卫生—药学]