人乳头瘤病毒感染与非小细胞肺癌发生的相关性  

Relationship between HPV Infection and Non-small Cell Lung Cancer

作　　者：姜蕊[1] 吴翠环[1] 郑丽端[2] 赵时宇[1] 陈多恩[1] 

机构地区：[1]华中科技大学同济医学院基础医学院病理学系,武汉430030 [2]华中科技大学同济医学院附属协和医院病理科,武汉430022

出　　处：《华中科技大学学报（医学版）》2005年第2期141-144,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

摘　　要：目的初步探讨人乳头瘤病毒(human papillomavirus, HPV)感染与非小细胞肺癌(non-small cell lung cancer,NSCLC)发生的相关性.方法用通用型引物PCR检测60例NSCLC和30例肺良性病变组织中HPV的感染率;用分型引物对阳性标本进行HPV16,18分型.用免疫组化方法检测 P53及Rb蛋白在肺癌组织中的表达.结果 60例NSCLC及30例良性病变组织中HPV的检出率分别为21例(35.0%)和1例(3.3%),二者差异具有显著性意义 (P<0.05).肺鳞癌HPV感染率(48.6%)显著高于肺腺癌(13.0%)(P<0.05),早期(Ⅰ期)肺癌HPV感染率(48.6%)显著高于进展期(Ⅱ～Ⅲ期,18.5%)(P<0.05),吸烟组HPV感染率(47.4%)显著高于非吸烟组(13.6%)(P<0.05).60例肺癌组织中34例P53蛋白阳性(56.7%),42例Rb蛋白阳性(70%),二者的表达在HPV感染阳性组和阴性组无显著性差异.结论 HPV感染在NSCLC的发生中可能起到一定的作用.Objective To investigate the relationship between human papillomavirus (HPV) infection and the pathogenesis of non-small cell lung cancer (NSCLC).Methods By using general polymerase chain reaction (PCR),the HPV positive rate and HPV gene type were determined in 60 cases of NSCLC and 30 cases of lung benign diseases.Immunohistochemistry was used to detect the expression of P53 and Rb in lung cancer tissue.Results HPV DNA was detectable in 35.0 % of NSCLC specimens and 3.3 % of benign diseases specimens by PCR with the difference being significant between them (P<0.05). HPV positive rate of squamous cell carcinoma (48.6 %) was significantly higher than that in adenocarcinoma (13.0 %,P<0.05) and that of early stage (48.5 %) was significantly higher than that in advanced stage (18.5 %,P<0.05). HPV positive rate in smoking group (47.4 %) was significantly higher than that in non-smoking group (13.6 %,P<0.05). The positive rate of P53 and Rb in 60 cases of lung cancer were 56.7 % and 70 % respectively. There was no significant difference in the expression of them between HPV positive group and HPV negative group.Conclusion HPV infection may play an important role in the pathogenesis of NSCLC.

关 键 词：人乳头瘤病毒 P53蛋白 RB蛋白 非小细胞肺癌 

分 类 号：R734.2[医药卫生—肿瘤]