几丁糖促进兔角膜碱烧伤愈合的作用  

Chitosan Promoting the Healing of Corneal Alkali Burn in Rabbits

作　　者：杨红[1] 向艳[1] 顾其胜 王蓉[1] 张晓农[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院眼科,武汉430030 [2]上海其胜生物制剂有限公司,上海201106

出　　处：《华中科技大学学报（医学版）》2005年第2期245-248,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：湖北省卫生厅科技计划项目基金资助 (No Lg200102)

摘　　要：目的　观察2%医用几丁糖在兔角膜碱烧伤早期对角膜上皮愈合, 抗过氧化损伤和炎症反应的影响。方法用15只新西兰大白兔制作角膜碱烧伤模型, 设立几丁糖组(右眼) 和磷酸盐缓冲液对照组(左眼)。裂隙灯观察角膜烧伤后角膜炎症反应及上皮修复过程。于烧伤后第7, 14, 28天各随机处死5只兔, 取角膜和房水检测超氧化物歧化酶(SOD) 活力和丙二醛(MDA) 含量, 并观察角膜组织病理学变化。结果　医用几丁糖组较对照组同期角膜上皮修复快, SOD活性高, MDA生成量少, 角膜炎症反应轻。结论　几丁糖在角膜碱烧伤急性期治疗中具有较强的促进上皮修复, 抗过氧化损伤及抑制炎症的作用。Objective To observe the effects of chitosan on corneal epithelial healing,lipid peroxidation and inflammation reaction in the early corneal alkali burn.Methods Corneal alkali burn model of New Zealand white rabbits was made. The rabbits were divided into chitosan group and control group. The healing of corneal epithelium and the inflammatory reaction of cornea were observed by slit lamp. On the 7th,14th and 28th day after burn,the activity of SOD and the content of MDA in the corneal and aqueous humor were detected. The corneal histopathology was observed under a microscope.Results In chitosan group,the healing of cornea epithelium was faster,the inflammatory reaction was milder,SOD activity was stronger and the content of MDA was lower than in control group.Conclusion Chitosan has strong effect on promoting corneal epithelial healing,decreasing lipid peroxidation damage and inhibiting inflammatory reaction.

关 键 词：角膜碱烧伤 愈合 几丁糖 抗过氧化损伤 炎症反应 

分 类 号：R772.2[医药卫生—眼科]