脑微血管内皮细胞体外缺血再灌注模型的建立及牛胆酸的保护作用  

The protective effect of cholic acid on an in vitro injury model of ischemia-reperfusion in cerebral microvascular endothelial cells

作　　者：高永红[1] 袁拯忠[1] 牛福玲[2] 崔巍[2] 朱陵群[1] 王硕仁[2] 

机构地区：[1]北京中医药大学东直门医院脑病实验室,北京100700 [2]北京中医药大学中医内科学教育部重点实验室,北京100700

出　　处：《中国中药杂志》2005年第12期930-932,共3页China Journal of Chinese Materia Medica

基　　金：国家重点基础研究发展规划项目课题 (G1999055404)

摘　　要：目的:建立脑微血管内皮细胞体外缺血再灌注模型,并观察了牛胆酸对其影响。方法:体外培养大鼠脑微血管内皮细胞,以氧糖剥夺(Krebs液)再复氧复糖模型模拟缺血再灌注损伤,并用牛胆酸进行了干预,用MTT比色法测定A值代表细胞生存活性。结果:氧糖剥夺4h再复氧复糖12h可造成培养大鼠脑微血管内皮细胞损伤,牛胆酸干预后A值明显高于模型组(P<0.01)。结论:牛胆酸可显著减轻脑微血管内皮细胞体外模拟缺血再灌注细胞损伤。Objective: To establish an in vitro injury model of ischemia-reperfusion in cerebral microvascular endothelial cells of rats and observe the protective effect of cholic acid. Method: Cultured rat microvascular endothelial cells were subjected to the oxygen-glucose deprivation (OGD) (Krebs solution) and recovery of oxygen-glucose, which simulated in vitro ischemia and reperfusion injury,and treated with cholic acid. The A value was measured with MTT chromatometry. Result: Cultured cells were impaired after OGD for 4 hours and recovery of oxygen-glucose for 12 hours, the A value of the cells treated with cholic acid was significantly higher than that of the cells without treatment(P<0.01). Conclusion: Cholic acid could obviously protect rat cerebral microvascular endothelial cells from injury induced by an in vitro ischemia-reperfusion.

关 键 词：脑微血管内皮细胞 大鼠 缺血再灌注 牛胆酸 

分 类 号：R743.3[医药卫生—神经病学与精神病学]