酶联免疫法在贝类麻痹性贝毒检测中的应用  

Enzyme - linked immunosorbent assay(ELISA) quantitative analysis for paralytic shellflsh poison (PSP) in shellflsh

作　　者：张纹[1] 王军[1] 苏永全[1] 

机构地区：[1]厦门大学海洋学系,福建厦门361005

出　　处：《海洋科学》2005年第6期35-37,共3页Marine Sciences

基　　金：厦门市海洋与渔业局资助项目

摘　　要：采用酶联免疫法检测菲律宾蛤仔(Ruditapes philippin)肌肉中麻痹性贝毒(Paralytic shellfish poison,PSP)含量,通过优化实验条件,规范操作程序,确立了检测流程。试验表明以标准PSP 为参照,该检测方法平均灵敏度可达2μg/kg,标准溶液测定的变异系数2.00%-7.66%,样品精密度测试的变异系数为2.82%-8.40%,平均添加回收率达85.35%,表现出快速、灵敏、可靠等特点,适于常规工作中麻痹性贝毒的快速筛选检测。Quantitative analysis of Paralytic Shellfish Poison (PSP) in Ruditapes philippin was carried with Enzyme -Linked Immunosorbent Assay (ELISA) . It showed that: the mean detection limit was 2μg/ kg, mean recovery rate was 85.35%, and coefficients of variation of 2.82% - 8. 40% indicated that the screening method is rapid, reliable, and sensitive.

关 键 词：麻痹性贝毒 酶联免疫法 快速检测 菲律宾蛤仔(Ruditapes philippin) 

分 类 号：TS254.7[轻工技术与工程—水产品加工及贮藏工程]