竞争性抑制剂对反胶团萃取过程中α胰凝乳蛋白酶活力的稳定作用  

Enhanced Activity Recovery of α-Chymotrypsin during Reverse Micelles Extraction by Addition of Competitive Inhibitor

作　　者：刘俊果[1,2] 沈睿[1] 邢建民[1] 阳承利[1] 刘会洲[1] 

机构地区：[1]中国科学院过程工程研究所分离科学与工程实验室 [2]河北科技大学生物科学与工程学院,河北石家庄050018

出　　处：《过程工程学报》2005年第3期260-264,共5页The Chinese Journal of Process Engineering

基　　金：国家基金委创新研究群体科学基金资助项目(编号:20221603)

摘　　要：研究了竞争性抑制剂苯基硼酸对α胰凝乳蛋白酶反胶团萃取过程的影响及对酶活力的稳定作用.研究发现,向AOT/isooctane反胶团中添加一定量的苯基硼酸,萃取率会略有增加,反萃取率则无显著变化.酶活力的回收率显著提高,酶活力对萃取和反萃时间的耐受性显著增强.苯基硼酸和α胰凝乳蛋白酶之间的特异性亲和作用是产生这种影响的原因.The effect of phenylboronic acid, a competitive inhibitor, on the extraction process and activity recovery of α-chymotrypsin by AOT[sodium 1,4-bis(2-ethylhexyl) sulfusuccinate] reverse micelles was studied. A moderate increase of extraction and in significant change of stripping were found when 2 mmol/L phenylboronic acid was added into the AOT reverse micelles system. The profound effect was that the activity recovery was greatly enhanced. The stability of enzyme activity was time dependent: the addition of phenylboronic acid induced enhanced activity recovery in a prolonged contact time during extraction and stripping. The specific binding of phenylboronic acid to chymotrypsin was considered to account for the stabilizing effect of enzyme activity during reverse micelles extraction.

关 键 词：反胶团萃取 竞争性抑制剂 α胰凝乳蛋白酶 苯基硼酸 

分 类 号：TQ925.6[轻工技术与工程—发酵工程]