酶转化异丁香酚制备香草醛的反应分离耦合方法  

Study on Simultaneous Reaction and Separation of Vanillin Prepared from Isoeugenol by Enzymatic Conversion

作　　者：王丰收[1] 赵丽青[1] 孙志浩[1] 

机构地区：[1]江南大学生物催化研究室,工业生物技术教育部重点实验室,江苏无锡214036

出　　处：《过程工程学报》2005年第3期273-276,共4页The Chinese Journal of Process Engineering

基　　金：国家973规划基金资助项目(编号:2003CB716008)

摘　　要：大豆脂氧合酶粗酶转化异丁香酚制备香草醛的过程中存在产物抑制酶转化现象,吸附剂HD?8树脂可以有效解除产物抑制,提高转化率.确定了加入底物量1.5%(?)、树脂量150g/L的优化转化工艺,使反应与分离进行很好的耦合,既提高了转化率,又能较好地实现底物和产物的分离.在优化条件下香草醛最高转化量可达3.41g/L,相应的摩尔转化率为24.53%.树脂洗脱法提取的产物得率为67.11%,底物的回收率为71.88%.There is product inhibition in the bioconversion from isoeugenol to vanillin by crude enzyme extracted from soybean. The product inhibition can be prevented effectively with the addition of resin HD-8. The optimum concentration of substrate is 1.5% (?). With 150 g/L HD-8 added at the beginning, the vanillin concentration reached its maximum (3.41 g/L), corresponding to a molar conversion rate of 24.53%. As a result, the substrate and product can be separated efficiently. The yield of product and the recovery of substrate are 67.11% and 71.88% respectively.

关 键 词：酶法转化 香草醛 异丁香酚 反应分离耦合 

分 类 号：Q814.9[生物学—生物工程]