酸胁迫作用下悬浮培养红豆杉细胞的磁场生物学效应  

Bio-effect of Magnetic Field on Suspension-cultured Taxus chinensis var. mairei under Acid Stress

作　　者：范寰[1] 商桂敏[1] 元英进[1] 

机构地区：[1]天津大学化工学院制药工程系,天津300072

出　　处：《过程工程学报》2005年第3期345-348,共4页The Chinese Journal of Process Engineering

基　　金：国家自然科学基金资助项目(编号:20236040)

摘　　要：研究了直流稳恒磁场对酸胁迫作用下悬浮培养南方红豆杉细胞生长和代谢的影响.结果表明,磁场提高了红豆杉细胞的抗酸胁迫能力,细胞活力上升,生物量和蛋白含量明显升高,胞外多酚氧化酶(PPO)活力升高,酚类物质积累受到抑制.在一定浓度的盐酸作用下细胞受到的酸胁迫越大,磁场的生物学效应越明显.胞外pH值和电导率的变化说明磁场提高细胞抗酸胁迫的能力与其加速了物质的跨膜转运有关.The effect of direct current magnetic field (DCMF) on the cell growth and metabolism of suspension-cultured Taxus chinensis var. mairei under acid stress was examined. The experimental results showed that the ability of cell anti-acid stress, cell viability, biomass and intracellular protein were obviously enhanced by the DCMF. The viability of extracelluar PPO was enhanced, and accumulation of phenolics was restrained. Under certain HCl concentration, the higher the concentration of HCl, the stronger the bio-effect of the magnetic field. The obvious change of the medium pH and conductivity indicated that the ability of cell anti-acid stress was enhanced due to accelerated material transmembrane transportation by DCMF.

关 键 词：直流稳恒磁场 南方红豆杉细胞 酸胁迫 跨膜运输 

分 类 号：Q64[生物学—生物物理学]