人卵巢癌细胞系具有不同转移潜能亚系的分离及体内和体外鉴定  

Isolation and Characte rization of Human O varian Cancer Cell Line Subclones with Diff erent Metastatic Potential

作　　者：陈洁[1] 刘鸣[1] 汤春生[1] 

机构地区：[1]山东大学山东省立医院,山东济南250021

出　　处：《实用妇产科杂志》2005年第6期350-352,共3页Journal of Practical Obstetrics and Gynecology

基　　金：山东省中青年优秀科学家科研奖励基金(编号01BS56)

摘　　要：目的:克隆具有不同转移潜能的癌细胞亚系,并对其侵袭转移能力进行体内外鉴定。方法:应用有限稀释法对卵巢癌细胞系SKOV3进行单细胞克隆,共分离出14个克隆亚系,根据细胞电泳率,流式细胞仪检测所得细胞周期,筛选出3个电泳率差异较大的克隆亚系(S1、S6和S14)。并应用体内外试验检测各亚系的生物学特性及其侵袭转移能力。结果:S1为高转移亚系,体外生长快,侵袭人工基底膜细胞数及软琼脂集落形成数显著多于其他二者,裸鼠体内100%成瘤,70%转移;而S14为不转移亚系,体外生长慢,侵袭人工基底膜细胞数及软琼脂集落形成数显著少于其他二者,裸鼠体内仅20%成瘤,无1例发生转移。结论:该系统为来自同一肿瘤母系,遗传背景完全相同,却具有不同转移潜能的异质性克隆,为肿瘤转移机制的研究和克隆肿瘤转移相关基因提供了良好模型。Objective:To is olate and characterize cancer cell subclones wit h differen t metastatic potential from human ovarian c ancer cell line. Methods:Using cell clone tech nique,cell elect rophoresis and flow cytometer,we establish ed three sublines with different metast atic potential from human ovarian cancer li ne-SKOV3 (S1,S6 and S14).The invasi veness and migratory capacity among the thr ee subgroups were observed by vitro g rowth,Matrigel invasion assay,soft agar cl oning and in viv o tumorigenicity and spontaneous metastasi s assay in nude mice. Results:Among the three subcl ones,S1 expressed as the highly metastatic phenotype,with 70 % metastasis frequency 6 weeks after subcutaneous inoculation into nude mice,wh ereas S14 was not metastatic.In vitr o,S1 was found to be the most highly invasiv e cell line in Matrigel invasive as say and had the most clones in soft agar clo ning assay.While,S1 4 was found to have the least invasive abili ty and the least clones. Conclusions:Successful estab lishment of thes e subclones with different metastatic pote ntial may be valuable for further stud y on the molecular mechanisms of cancer met astasis and cloning of cancer metastasis related g enes. [

关 键 词：卵巢肿瘤 肿瘤浸润 细胞系 

分 类 号：R737.31[医药卫生—肿瘤]