津白Ⅱ小鼠自发乳癌（ⅥTA_2MA－891）高自发肺转移模型  

A Murine Model of Mammary Adenocarcinoma(Ⅵ TA_2 MA-891)with High Rate of Spontaneous Metastases in the Lungs

作　　者：高朋根[1] 苏蕴 高友春[1] 刘习昌 王继信[1] 张友会[1] 

机构地区：[1]中国医学科学院,中国协和医科大学肿瘤研究所

出　　处：《中国医学科学院学报》1994年第2期147-152,共6页Acta Academiae Medicinae Sinicae

摘　　要：本模型获自我组１只３４８日龄雌性津白Ⅱ小鼠右侧第五乳腺处一肿物。病理切片检查为乳腺腺癌，属于Ｄｕｎｎ（１９５９）分类之Ｂ型。用本组同源小鼠（ｉｓｏｇｅｎｅｉｃｍｉｃｅ）皮下移植已传到２７代。连续皮下移植成瘤率为１００％，未见自然消退；潜伏期４～７ｄ；肺转移瘤结多发而密集；最早转移时间，肉眼观察为接种后第３５天，镜检为第１０天。肺自发转移率，１１～２０代为１００％。皮下移植瘤、肺转移瘤之病理组织结构及其癌细胞形态与其原发瘤近似。带瘤鼠濒死时其皮下移植瘤体积平均值为４．９３ｃｍ３。７种抗癌药对皮下移植瘤抑制率，氟尿嘧啶为２９．７５％（Ｐ＜０．０５）、塞替派为４４．８８％（Ｐ＜０．０１）、环磷酰胺９５．３１％（Ｐ＜０．０１）、平阳霉素９６．７５％（Ｐ＜０．０１），其它三药抑制率均不足３０％。对肺转移瘤抑制率，平阳霉素为４２．５４％（Ｐ＜０．０１），余不足３０％。The primary donor tumor was a spontaneous mammary tumor which arose in a 348-dayold female TA_2 mouse.The pedigree number of that mouse was 0901-1423.The mouse was sacrificed for serial s.c.transplantation with 3 isogeneic hosts on January 19,1989 and a total of 27 generations succeeded by the end of April,1992.The primary donor tumor was diagnosed pathologically as a type B mouse mammary adenocarcinoma(Dunn 1959).The incidence of this serial s.c.transplantation tumor is 100% after inoculation.The latency period is 4-7 days and the lifetime of tumorbearing mice is about 47 days.The spontaneous metastatic rates in the lugs was 100% after the 11th generations.The earliest metastasis seen in the lung under light microscopy was on the 10th day after inoculation,and the earliest seen by gross examination was on about the 35th day.The transplanted tumor grew slowly from the 4th day to the 21 st day after inoculation and then grew rather fast from the 22nd to the 47th day.The average size of the transplanted tumor obtained from 10 recipient mice on the 47th day after inoculation was 4.93cm3.Seven anticancer drugs were administered respectively for a sensitivity test.The taking rates of the transplanted tumor using 4 of the 7 drugs were as follows: 5-fluorouracil 29.27%(P<0.05),thio-tepa 44.80%(P<0.01);cyclophosphamide 95.31%(P<0.01);and bleomycin 96. 27%(P<0.01).Only bleomycin was able to significantly inhibit lung metastasis,with an inhibition rate of 42.54%(P<0.01).In conclusion,the pathological characteristics of the transplanted tumor and its metastasis in the lungs resemble those of the primary donor tumor.

关 键 词：小鼠 移植 肺转移 乳腺癌 

分 类 号：R737.9[医药卫生—肿瘤]