马铃薯Y病毒N株HC-pro基因的序列分析与表达  

Sequence Analysis of the HC-pro Gene of Potato Virus Y N Strain and its Expression in E .coli

作　　者：王睿[1] 吴云锋[1] 郝兴安[1] 付宏岐[1] 杨栋[1] 樊兵[1] 

机构地区：[1]西北农林科技大学植保学院陕西省农业分子生物重点实验室,陕西杨凌712100

出　　处：《中国病毒学》2005年第3期320-322,共3页Virologica Sinica

基　　金：国家自然科学基金(30270876)

摘　　要：马铃薯Ｙ病毒(Potato virus Y,PVY)是马铃薯Ｙ病毒属(Potyvirus)的典型成员,是烟草、马铃薯和辣椒等茄科作物病毒病的主要病原之一,分布于世界各地.近年烟草上的 PVY在全国范围内特别是陕西、黄淮和东北烟区流行呈上升趋势,且以PVY坏死株系（PVY-N)为主,重病田发生率达40%以上,并常与烟草花叶病毒、黄瓜花叶病毒混合侵染,严重影响烟草品质造成巨大的经济损失.The HC-pro gene was amplified by RT-PCR from total RNA of tobacco leaves infected with a N strain of Potato virus Y in Shaanxi, and cloned into the PMD 18-T vector. This HC-pro gene is consisted of 1371 nucleotides, encoding 457 amino acids. It shared the sequence homology of 82.5%~96.4 % nucleotide acid and 92.5%~98.0% in amino acids compared to 9 species of PVY N HC-pro abroad.The HC-pro gene was inserted into prokaryotic expressing vector pBV221, to obtain pBVHC recombinant plasmid in E.coli BL21. SDS-PAGE indicated that HC-pro proteins are successfully expressed in E.coli, Western blotting analysis demonstrated that the antibody against the expressed HC-pro can be used to identify the infected plants .

关 键 词：HC-PRO基因 马铃薯Y病毒 序列分析 表达 

分 类 号：S432.41[农业科学—植物病理学]