Oncogenic role of clusterin overexpression in multistage colorectal tumorigenesis and progression  

作　　者：DanXie JonathanS.T.Sham Wei-FenZeng Li-HongChe MengZhang Hui-XiWu Han-LiangLin Jian-MingWen SzeHangLau LiangHu Xin-YuanGuan 

机构地区：[1]DepartmentofPathology,ZhongShanMedicalCollege,SunYat-SenUniversity,Guangzhou510089,GuangdongProvince,China [2]DepartmentofClinicalOncology,TheUniversityofHongKong,HongKong,China

出　　处：《World Journal of Gastroenterology》2005年第21期3285-3289,共5页世界胃肠病学杂志（英文版）

基　　金：Supported by the Natural Science Foundation of China, No. 30300401,Guangdong Natural Science Foundation, No. 04009327the Leung Kwok Tze Foundation of Hong Kong, China

摘　　要：AIM: To investigate the expression pattern of clusterin in colorectal adenoma-carcinoma-metastasis series,and to explore the potential role of clusterin in multistage colorectal tumorigenesis and progression. METHODS: A colorectal carcinoma (CRC)-tissue microarray (TMA), which contained 85 advanced CRCs including 43 cases of Dukes B,21 of Dukes C and 21 of Dukes D tumors, were used for assessing the expression of clusterin (clone 41D) and tumor cell apoptotic index (AI) by immunohist-ochemistry and TUNEL assay,respectively.Moreover the potential correlation of dusterin expression with the patient's clinical-pathological features were also examined. RESULTS: The positive staining of clusterin in different colorectal tissues was primarily a cytoplasmic pattern. Cytoplasmic overexpression of clusterin was detected in none of the normal colorectal mucosa, 17% of the adenomas, 46% of the primary CRCs, and 57% of the CRC metastatic lesions. In addition, a significant positive correlation between overexpression of clusterin and advanced clinical (Dukes) stage was observed (P<0.01).Overexpression of cytoplasmic clusterin in CRCs was inversely correlated with tumor apoptotic index (P<0.01),indicating the anti-apoptotic function of cytoplasmic clusterin in CRCs. CONCLUSION:These data suggests that overexpression of cytoplasmic dusterin might be involved in the tumorigenesis and/or progression of CRCs. The anti-apoptotic function of cytoplasmic dusterin may be responsible, at least in part, for the development and biologically aggressive behavior of CRC.AIM: To investigate the expression pattern of clusterin in colorectal adenoma-carcinoma-metastasis series, and to explore the potential role of dusterin in multistage colorectal tumorigenesis and progression.METHOD:S: A colorectal carcinoma (CRC)-tissue microarray (TMA), which contained 85 advanced CRCs including 43 cases of Dukes B, 21 of Dukes C and 21 of Dukes D tumors, were used for assessing the expression of clusterin (clone 41D) and tumor cell apoptotic index (AI) by immunohistochemistry and TUNEL assay, respectively. Moreover the potential correlation of dusterin expression with the patient'sclinical-pathological features were also examined. RESULTS: The positive staining of clusterin in different colorectal tissues was primarily a cytoplasmic pattern. Cytoplasmic overexpression of clusterin was detected in none of the normal coloredal mucosa, 17% of the adenomas, 46% of the primary CRCs, and 57% of the CRC metastatic lesions. In addition, a significant positive correlation between overexpression of clusterin and advanced clinical (Dukes) stage was observed (P＜0.01). Overexpression of cytoplasmic clusterin in CRCs was inversely correlated with tumor apoptotic index (P＜0.01), indicating the anti apoptotic function of cytoplasmic clusterin in CRCs.CONCLUSION: These data suggests that overexpression of cytoplasmic dustin might be involved in the tumorigenesis and/or progression of CRCs. The anti-apoptotic function of cytoplasmic dusterin may be responsible, at least in part, for the development and biologically aggressive behavior of CRC.

关 键 词：CLUSTERIN Colorectal tumorigenesis 

分 类 号：R735[医药卫生—肿瘤]