视网膜脱离神经节细胞存活与IL-1β关系的实验研究  

Retinal ganglion cell survival and IL-1β level in experimental retinal detachment

作　　者：卢华[1] 马志中[2] 刘敬[1] 曹立群[1] 

机构地区：[1]解放军总医院眼科,北京100853 [2]北京大学第三医院北大眼科中心,100083

出　　处：《眼科研究》2005年第3期286-289,共4页Chinese Ophthalmic Research

摘　　要：目的探讨在视网膜脱离及复位状态下白介素1β(IL1β)对神经节细胞(RGC)的存活数的影响,检测白介素1受体拮抗物(IL1Ra)对RGC的保护作用。方法SD大鼠用1%荧光金经上丘、外侧膝状体逆行标记,1.4%HealonGV鼻侧视网膜下注射。在逆标后10d,脱离后的不同时间(或复位后的不同时间)行视网膜铺片,在荧光显微镜下行RGC计数。并对不同时间点的视网膜内的IL1β含量用放射免疫方法进行检测。结果视网膜脱离后2hRGC即有丢失,1d时达到高峰后缓慢下降。未脱离区亦有RGC的丢失。视网膜复位可以停止RGC的丢失。IL1β抗体、IL1Ra可以减少这种丢失。IL1β在脱离后第7d达到高峰后下降。结论视网膜脱离导致RGC的丢失。早期复位可使其丢失停止。IL1Ra对RGC有保护作用。IL1β是脱离状态下RGC丢失的关键因素。ObjectiveTo determine the effect of IL-1β on the retinal ganglion cell survival during retinal detachment and reattachment and explore the neuoprotection of IL-1Ra.MethodsRetina of Sprague-Dawley (SD) rat was examined after subretinal injection of 1.4%Healon GV and retrograde labeling using flurogold (FG) at different time.Healon GV was combined with 10ng IL-1Ra and 500ng IL-1beta antibody respectively and then injected into the subretinal space at different time points.Levels of IL-1β in detached and reattached retina were detected by radio-immunity method.ResultsRGCs degeneration occurred 2 hour after detachment and reached its peak at 1 day.RGCs degeneration also was found in the opposite area of the peripheral attached retina.Loss of RGCs could be stopped by retinal reattachment,and subretinal space injection of IL-1Ra and IL-1beta antibody decreased RGCs loss in detached retina.IL-1β reached a highest level at the 7th post-injection day and then declined gradually and remained a stable level.ConclusionRetinal detachment results in RGCs loss.Loss of RGCs is existed both in the detached and attached retina.IL-1Ra has a neuroprotective effect on detached-retina,and IL-1β is a key factor of RGCs loss.

关 键 词：神经节细胞 视网膜脱离 视网膜复位 白介素-1Β 白介素-1受体拮抗物 

分 类 号：R774[医药卫生—眼科]