Clusterin、bcl-2及FasL在前列腺癌中的表达及相关性研究  

Expression and significance of Clusterin ,bcl-2 and FasL in prostate cancer

作　　者：陈刚[1] 唐伟[1] 

机构地区：[1]重庆医科大学临床学院泌尿外科,重庆400016

出　　处：《重庆医科大学学报》2005年第3期409-411,420,共4页Journal of Chongqing Medical University

摘　　要：目的:通过检测新的凋亡抑制因子Clusterin在前列腺癌组织中的表达,探讨其与前列腺癌发生发展的关系及其与bcl-2和FasL表达的关系。方法:A采用免疫组织化学染色法检测Clusterin、bcl-2、FasL在10例正常前列腺组织、15例良性前列腺增生组织(BPH)、49例前列腺癌组织标本中的表达。结果:Clusterin在前列腺正常、增生、癌组织中的阳性及弱阳性表达率分别为10%(1/10)、66.6%(10/15)、91.8%(45/49)。前列腺癌组织中Clusterin表达水平明显高于前列腺正常组织(P<0.005)及增生组织(P<0.005),且在癌组织中与肿瘤临床分期(P<0.001)、病理分级(P<0.001)呈正相关。比较Clusterin与bcl-2在前列腺癌组织中的表达,差异有显著性(P<0.05)。比较Clusterin与FasL在前列腺癌组织中的表达,差异也有显著性(P<0.05)。结论:Clusterin基因可能通过抑制凋亡在前列腺癌的发生发展中发挥重要作用,且其表达与bcl-2、FasL的异常表达密切相关。Objective:To investigate the expression and significance of an inhibitor gene of apoptosis,Clusterin,in prostate cancer,and its relationship with the expression of bcl-2,FasL.Methods:The expression of Clusterin,bcl-2 and FasL in sample of 10 normal prostate,15 BPH and 49 prostate cancer were examined by immunohistochemical stain.Results:The rate of positive or weak positive for normal prostate,BPH and prostate cancer was 10%(1/10),66.6%(10/15)and 91.8%(45/49) respectively.The expression level of Clusterin in prostate cancer was much higher than in normal prostate(P<0.005),BPH(P<0.005),and it was related positively with clinical stage(P<0.001)and pathological grade(P<0.001)of prostate cancer.Clusterin positive cases were strongly associated with bcl-2 expression(P<0.05)and FasL expression(P<0.05).Conclusion:Clusterin,alone or in cooperarion with bcl-2 and FasL,may play an important role in the onset and progression of prostate cancer by the antiapoptosis pathway.

关 键 词：前列腺肿瘤 凋亡 CLUSTERIN FASL BCL-2 免疫组织化学 

分 类 号：R737.25[医药卫生—肿瘤]