外源性透明质酸酶对人乳癌细胞株体外增殖的影响  

The effect of exogenous hyaluronidase on proliferation of human breast cancer cells in vitro

作　　者：王良[1] 任国胜[1] 涂刚[1] 

机构地区：[1]重庆医科大学临床学院普外科,重庆400016

出　　处：《重庆医科大学学报》2005年第3期442-443,共2页Journal of Chongqing Medical University

基　　金：国家自然科学基金资助项目(30371393)

摘　　要：目的:探讨外源性透明质酸酶(Hyaluronidase,Hyase)对人乳癌细胞株增殖的影响。方法:应用MTT法和流式细胞仪观察外源性Hyase对人乳癌细胞株增殖率及细胞周期分布的影响。结果:ZR-75-30细胞株实验组A值(0.674±0.221)明显高于对照组(0.563±0.046),P<0.05;实验组S期细胞百分率(%)为18.36±0.65,对照组为2.19±0.10;实验组G0/G1期细胞百分率(%)为44.49±4.33,对照组为62.14±26.97,两组比较均有显著性差异(P<0.05)。MDA-MB-435细胞株实验组A值(0.694±0.054)明显高于对照组(0.578±0.072),P<0.05。MDA-MB-231细胞株实验组A值(0.736±0.059)明显高于对照组(0.637±0.046),P<0.05。结论:外源性Hyase可能具有促进人乳癌细胞株增殖的作用。Objective:To study the effect of exogenous hyaluronidase on proliferation of human breast cancer cells in vitro.Methods:The proliferation of human breast cancer cell line ZR-75-30 were detected by MTT-assay and flow cytometry.Results:The absorbency value(A) of ZR-75-30 in study group treated with Hyase (0.674±0.221) was significantly higher than that in control group(0.563±0.046).The percentage of phase S cells in study group(18.36±0.65) was higher than that in control group(2.19±0.10);the percentage of phase G0/G1 cells in study group(44.49±4.33) was lower than that in control group(62.14±26.97).The absorbency value(A) of MDA-MB-435 in study group (0.674±0.221) was significantly higher than that in control group(0.563±0.046).The absorbency valve(A) of MDA-MB-231 in study group (0.674±0.221) was significantly higher than that in control group(0.563±0.046).Conclusion:Exogenous hyaluronidase may promote the proliferation of breast cancer cell lines in vitro.

关 键 词：透明质酸酶 乳癌 细胞周期 

分 类 号：R737.9[医药卫生—肿瘤] R737.2[医药卫生—临床医学]