年龄对大鼠多器官衰竭模型肺泡巨噬细胞炎症反应的影响  

Effects of age on alveolar macrophages in rats with multiple organ failure

作　　者：钱小顺[1] 朱庆磊[1] 杨 尹彤[1] 丁秀云[1] 王士雯[1] 

机构地区：[1]解放军总医院老年心血管病研究所

出　　处：《中华老年医学杂志》2005年第5期373-375,共3页Chinese Journal of Geriatrics

基　　金：国家重点基础研究发展规划资助项目(G2000057004)

摘　　要：目的　探讨年龄对大鼠多器官衰竭模型肺泡巨噬细胞产生炎性介质能力的影响。　方法　用酵母多糖复制老年和非老年大鼠多器官衰竭模型,通过支气管肺泡灌洗和细胞贴壁的方法获取巨噬细胞,用放射免疫分析法(RIA)进行血清和巨噬细胞培养上清液中肿瘤坏死因子(TNF α)和白细胞介素10(IL 10)的检测。　结果　两个模型组血清TNF α均高于对照组,而老年模型组血清TNF α为(2 57±0 78)μg/L,高于非老年模型组的(1 89±0 83)μg/L(P<0 05)。各模型组巨噬细胞培养上清液TNF α和IL 10水平高于对照组(P<0 05),但模型组之间差异无统计学意义。　结论　大鼠多器官功能衰竭模型中,老年大鼠模型的全身炎症反应大于非老年大鼠模型,但巨噬细胞在分泌炎性因子方面差别无统计学意义。Objective To investigate the function of alveolar macrophage (AM) effected by age in the models of rats with multiple organ failure(MOF) Methods Serums were obtained from the elder rat models with MOF at 24 hr after zymosan administration Supernant was obtained from AM which had been cultured in vitro for 20 hours Tumor necrosis factor (TNF a) and Interleukin 10 (IL 10) in serum and supernant were measured by RIA Results The levels of TNF α in serums of both model groups were higher than those of their control groups TNF α in serum of aged model(2 57±0 78)μg/L was higher than that of adult model(1 89±0 83)μg/L( P <0 05) TNF-α and IL-10 levels in supernant of both model groups were higher than those of their control groups But there was no difference between both model groups( P >0 05). Conclusions Systemic inflammation reactions in the aged model are more serious than those in the adult model But age has no effect on the function of alveolar macrophage.

关 键 词：肺泡巨噬细胞 多器官衰竭 白细胞介素-10(IL-10) 肿瘤坏死因子(TNF-α) 年龄 细胞培养上清液 血清TNF-α 老年大鼠模型 支气管肺泡灌洗 放射免疫分析法 多器官功能衰竭 全身炎症反应 炎性介质 酵母多糖 细胞贴壁 炎性因子 

分 类 号：R363[医药卫生—病理学]