DLC-1基因表达与肝细胞癌复发转移的关系  

Relationship between DLC-1 expressions and metastasis in hepatocellular carcinoma

作　　者：宋丽杰[1] 叶胜龙[1] 王凯峰[1] 翁永强[1] 梁春敏[1] 孙瑞霞[1] 赵燕[1] 刘银坤[1] 汤钊猷[1] 

机构地区：[1]复旦大学肝癌研究所,中山医院,教育部癌变与侵袭原理重点实验室,上海200032

出　　处：《中华肝脏病杂志》2005年第6期428-431,共4页Chinese Journal of Hepatology

基　　金：复旦大学创新基金(CQF152810)国家自然科学基金(30271459)国家重点基础研究(973)项目(G1998051210)

摘　　要：目的已报道人类染色体8p缺失可能与肝癌转移有关,本文应用实时定量聚合酶链反应(RQ- PCR)研究位于8p的DLC-1基因mRNA表达与肝细胞癌侵袭转移的关系。方法收集51例复旦大学中山医院外科手术切除的肝细胞癌(HCC)及癌旁正常组织标本,根据临床病理学指标,分为高低侵袭性两组,用RQ-PCR对不同侵袭性HCC之间的DLC-1基因表达进行分析。对不同侵袭转移潜能MHCC97-L、MHCC97-H、HCCLM3、Hep3B及HepG2肝癌细胞系用同样方法分析DLC-1基因的表达差异。结果非转移细胞系Hep3B和HepG2与转移细胞系MHCC97-L、MHCC97-H和HCCLM3之间DLC—1基因表达差异有统计学意义(P<O.01),并随着侵袭与转移潜能的逐步提高DLC-1表达水平也相应逐步降低;HCCLM3 细胞系显著低于MHCC97-L细胞系(P<0.01)。HCC组织中,高侵袭性HCC组DLC-1基因表达明显低于低侵袭性HCC组(P<0.05)。结论DLC-1基因表达与肝癌的侵袭转移的抑制相关,其表达可能在抑制肝癌的侵袭转移中发挥重要作用。Objective To study the relationship between the expression level of DLC-1 mRNA (located in 8p) and the invasion/metastasis of human hepatocellular carcinoma (HCC). Methods Fifty-one surgical specimens of human HCC were divided into high-invasive and low invasive groups according to their clinicopathological features. DLC-1 mRNA expression was studied in the 51 HCC specimens as well as 5 different metastasis potential cell lines using realtime quantitative PCR (RQ-PCR). Results The expression level of DLC-1 mRNA in HCC specimens with high invasiveness was significantly lower than that with low invasiveness (P < 0.05). The expression levels of DLC-1 mRNA were significantly different between non-metastatic (Hep3B and HepG2) and metastatic (MHCC97-H, MHCC97-L and HCCLM3) cell lines (P < 0.05). From MHCC97-L to HCCLM3, with an increase of invasiveness and metastatic potentials, the expression level of DLC-1 decreased correspondingly, and its expression level in HCCLM3 was significantly lower than that in MHCC97-L (P < 0.01). Conclusion The expression of DLC-1 mRNA may play an important role in inhibiting the invasiveness and metastasis of HCC.

关 键 词：肝细胞癌 复发转移 MHCC97-L MHCC97-H 实时定量聚合酶链反应 HCCLM3 基因mRNA表达 HepG2 Hep3B 侵袭转移 外科手术切除 RQ-PCR 基因表达差异 低侵袭性 转移潜能 染色体8P 病理学指标 肝癌细胞系 肝癌转移 组织标本 中山医院 

分 类 号：R735.7[医药卫生—肿瘤] R737.9[医药卫生—临床医学]