高效液相色谱法测定痰咳净中咖啡因的含量  

Determination of caffeine in Tankejing with high-performance liquid chromatography

作　　者：何明三[1] 汪经环[1] 雷永军 万君梅 

机构地区：[1]湖北中医学院中药系

出　　处：《中国医院药学杂志》1994年第9期391-393,共3页Chinese Journal of Hospital Pharmacy

摘　　要：本文报道了痰咳净中咖啡因的ＨＰＬＣ测定法。使用Ｚｏｒｂａｘ－Ｃ１８柱，流动相为甲醇－水（１：１），检测波长２５４ｎｍ，非那西丁为内标物。测定中其它组分无干扰，方法简便，重现性好，平均回收率为９９．６％，ＲＳＤ为０．５％。HPLC method for the determination of caffeine in Tankejing was described .The sample was analysed by a Zorbax-C18column with 50% methanol as mobile phase,using a UV-detector at 254nm,the internal standard was Phenacetin.Themethod was simple and accurate. The recovery of Caffeine was 99.6%and the variation coefficient was 0.5%.

关 键 词：痰咳净 咖啡因 高效液相色谱 

分 类 号：R927.2[医药卫生—药学]