重组人干扰素βser17工程菌发酵培养条件的优化研究  

Studies on the optimal fermentation conditions for preparation of recombinant human interferon-β ser17(rhIFN-β ser17)expressed by E.coli

作　　者：张振龙[1] 刘建源[1] 邓宛明[1] 郭秀梅[1] 郭鹏[1] 扬云凯 

机构地区：[1]北京生物制品研究所,北京100024 [2]北京天坛生物制品股份有限公司,北京100024

出　　处：《微生物学免疫学进展》2005年第2期21-23,共3页Progress In Microbiology and Immunology

摘　　要：实验对重组人干扰素βser17(rhIFNβser17)工程菌的发酵培养条件进行了研究,通过实验优化确定了适宜rhIFNβser17表达的培养基、诱导剂使用浓度、诱导时期和诱导后的收获时间等,建立了发酵培养工艺,并在50L发酵罐进行了中试发酵,菌体收获量湿重达13.08g/L,rhIFNβser17表达量占菌体总蛋白的20.2%,纯化后比活性达2×107IU/mg蛋白以上,为进一步的下游开发奠定了基础。The fermentation process of a expression E.coli system in preparation recombinant human interferon-β ser17(rhIFN-β ser17)was studed. Expression medium, IPTG concentration, induction time and harvest period were optimized. The optimal condition for these factors were determined. The fermentation process was scaled up to pilot scale on 50 L-fermenter. The bacterial wet weight could reach 13.08g/L culture and expression level of rhIFN-β ser17 was 20.2%. The specific activity of rhIFN-β ser17 reached above 2×10^(7)IU/mg protein.

关 键 词：重组人干扰素βser17 发酵 表达 

分 类 号：TQ464[化学工程—制药化工]