胃癌组织中uPA系统及VEGF表达与侵袭转移的关系  

Expression of urokinase-type plasminogen activator system and vascular endothelial growth factor in human gastric cancer and the relationship with tumor invasion and metastasis

作　　者：秦蓉[1] 盛霞[1] 吴继锋[1] 王道斌[1] 

机构地区：[1]安徽医科大学病理教研室,安徽省合肥市230032

出　　处：《世界华人消化杂志》2005年第6期706-710,共5页World Chinese Journal of Digestology

基　　金：安徽省教育厅资助项目;N0.2001kj128~~

摘　　要：目的:研究尿激酶型纤溶酶原激活系统uPA,uPAR, pAI-1蛋白及血管内皮生长因子(VEGF)在胃癌组织中的表达及其与胃癌生物学行为的关系. 方法:采用免疫组化S—P法检测67例胃癌及癌旁组织中uPA,uPAR,PAI-1及VEGF蛋白表达情况,同时结合临床生物学特征进行分析. 结果:癌和癌周比较uPA,uPAR,PAI-1及VEGF蛋白阳性表达率明显升高(P<0.01),阳性产物主要分布在癌细胞胞质中.侵及浆膜组阳性率明显高于未及浆膜组(P=0.000,0.014,0.016,0.000).uPA,uPAR 蛋白在淋巴结转移组中的阳性率明显高于无淋巴结转移组(P=0.002,0.008).PAI-1蛋白在低分化组中的阳性率明显低于中分化和高分化组(P<0.05).uPA,upAR 及VEGF蛋白阳性率在胃癌组织不同分化程度间差异无显著性.在胃癌组织中uPA,uPAR蛋白的表达呈显著正相关(r=0.653,P<0.001).在uPA,uPAR蛋白同时阳性的41例病例中,有28例发生淋巴结转移,与阴性组(4/15)相比,转移发生率明显增高(P<0.01).胃癌组织中uPA蛋白表达与VEGF表达显著正相关(r=0.300. P<0.05).PAI-1蛋白表达与VEGF表达具有极显著正相关性(r=0.413,P<0.01).应用COX回归模型进行多变量分析并采用向前逐步回归法,仅浸润深度、uPA表达和病理类型对淋巴结转移有影响. 结论:uPA、uPAR、PAI-1及VEGF蛋白在胃癌组织中表达增强,uPA,uPAR与胃癌浸润转移密切相关.AIM: To detect the expression of urokinase-type plasmi-nogen activator (uPA), uPA receptor (uPAR), plasminogen activator inhibitor type 1 (PAI-1) and vascular endothelial growth factor (VEGF) in human gastric cancer (GC) and to reveal their correlation to the major clinicopathological characteristics including tumor invasion and metastasis. METHODS: Expression of uPA, uPAR, PAI-1 and VEGF was examined in the tissue sections from 67 cases of human GC using streptoavidin-biotin-peroxidase immuno-histochemistry method. RESULTS: The expression of uPA, uPAR, PAI-1 and VEGF was mainly located in the cytoplasm of GC cells. The expression of uPA, uPAR, PAI-1 and VEGF in GC was significantly higher than in the cancer-adjacent tissue (P<0.01). The expression of uPA, uPAR, PAI-1 and VEGF in GC with serosa invasion was significantly higher than that in cancer without serosa invasion (P<0.05). Positive rates of staining for uPA and uPAR in GC with lymph node me- tastasis were significantly higher compared to cancer without lymph node metastasis (P = 0.002 and 0.008 respectively). The expression of RAM was significantly associated with the differentiation degree of cancer (P<0.01), while no relationship was found between the expression of uPA, uPAR, VEGF and the differentiation degree of cancer. The expression of uPA was correlated with uPAR in cancer tissue(r= 0.653, P<0.001). In 41 cases positive for uPA and uPAR, 28 were with lymph node metastasis (P<0.01, compared with 4/15). Positive correlation was observed between the expression of uPA and VEGF protein (r= 0.300, P<0.05), and between PAI-1 and VEGF (r= 0.413, P< 0.01). Multivariate Cox regression analysis indicated that only the depth of invasion, the expression of uPA and the differentiation affected lymph node metastasis in GC. CONCLUSION: Expression of uPA, uPAR, PAI-1 and VEGF protein is increased in gastric cancer. uPA and uPAR may play an important role in the invasion and metastasis through VEGF which promotes angiogenesis.

关 键 词：VEGF表达 胃癌组织 UPA系统 血管内皮生长因子(VEGF) 侵袭转移 尿激酶型纤溶酶原激活系统 VEGF蛋白 PAI-1 免疫组化S-P法 PAR蛋白 胃癌及癌旁组织 COX回归模型 uPAR 癌生物学行为 生物学特征 阳性表达率 逐步回归法 多变量分析 

分 类 号：R735.2[医药卫生—肿瘤]