D-氨基葡萄糖衍生物对人胃癌细胞系SGC-7901增生的影响  

Effect of amino-D-glucose derivative on proliferation of human gastric carcinoma cell line SGC-7901 in vitro

作　　者：吴静[1] 周芸[1] 路红[1] 王玉玲[1] 王爱勤[2] 薛群基[2] 

机构地区：[1]兰州大学第一医院消化科,甘肃省兰州市730000 [2]中国科学院兰州化学物理研究所,甘肃省兰州市730000

出　　处：《世界华人消化杂志》2005年第6期720-723,共4页World Chinese Journal of Digestology

摘　　要：目的:观察D-氨基葡萄糖衍生物体外对人胃癌SGC- 7901细胞增生的影响,探讨其可能作用机制. 方法:采用MTT法,筛选药物作用最佳浓度.用流式细胞仪分析诱导凋亡前后的细胞DNA含量,透射电镜观察凋亡细胞的超微结构.免疫组化测定CD44表达. 结果:D-氨基葡萄糖衍生物能明显抑制胃癌细胞的增长(P<0.01),MTT法显示抑制程度具有时间和剂量效应关系,统计组间比较差异有显著性(P<0.01);流式细胞仪分析可见亚二倍体(Sub-G1)凋亡峰;电镜观察到凋亡细胞的典型变化,免疫组化及光密度检测示CD44表达下降(216.5±7.0 vs 190.0±14.2,P=0.000). 结论:D-氨基葡萄糖衍生物通过诱导肿瘤细胞凋亡而抑制人胃癌SGC-7901细胞增生,同时还有下调CD44的作用.AIM: To study the in vitro inhibitory effect of amino-D-glu-cose derivative on the proliferation of the gastric carcinoma cell line SGC-7901. METHODS: Cell growth was measured by MTT colorimet-ric assay. DNA content was detected by flow cytometer (FCM). The morphological changes of apoptotic cells were evaluated by transmission electronic microscopy. CD44 expression was analyzed by immunohistochemistry. RESULTS: Amino-D-glucose derivative inhibited SGC-7901 cell in a time- and dose-dependent manner. After treatment, apoptosis peak was demonstrated by FCM, and apoptotic morphological changes were observed under microscope. Immunohistochemist and image analysis showed that expression of CD44 was downregulated (216.5±7.0 vs 190.0±14.2, P= 0.000). CONCLUSION: Amino-D-glucose derivative can induce SGC -7901 cell apoptosis, inhibit the cancer proliferation and downregulate expression of CD44 expression.

关 键 词：SGC-7901 D-氨基葡萄糖衍生物 人胃癌细胞系 流式细胞仪分析 CD44表达 细胞DNA含量 透射电镜观察 剂量效应关系 肿瘤细胞凋亡 细胞增生 MTT法 免疫组化 凋亡细胞 生物体外 作用机制 诱导凋亡 最佳浓度 药物作用 超微结构 

分 类 号：R735.2[医药卫生—肿瘤]