不同牧草品种对体外发酵产气特性的影响  

Effects of different forage species on in vitro gas production characteristics

作　　者：汤少勋[1] 姜海林[1] 周传社[1] 谭支良[1] 

机构地区：[1]中国科学院亚热带农业生态研究所,湖南长沙410125

出　　处：《草业学报》2005年第3期72-77,共6页Acta Prataculturae Sinica

基　　金：中国科学院知识创新重要方向项目(kzcx2-sw-415);国家"十五"攻关项目(2001BA508B03)资助。

摘　　要：采用改进的体外产气技术评价了10种不同牧草的体外产气特性。利用非线性回归方程获得了Gompertz模型的发酵动力学参数(理论最大产气量、产气速率常数和延滞时间),分析了不同品种牧草间发酵动力学参数的差异及发酵动力学参数与营养成分之间的相关关系。结果表明,不同品种牧草间产气动力学参数差异显著(P<0.05)。理论最大产气量、产气速率常数和产气延滞时间分别以苏丹草、黑麦草和桂牧1号最高,而以鲁梅克斯、鲁梅克斯和苏丹草最低。相关分析表明理论最大产气量和累积产气量分别与OM含量、NDS/CP值及(NDS-Ash)/CP值呈极显著正相关关系(P<0.001),而与CP含量呈极显著负相关关系(P<0.01)。产气速率常数与营养成分无显著相关性。产气延滞时间与半纤维素的含量呈极显著正相关关系(P<0.01),而与NDS/CP值和(NDS-Ash)/CP值呈极显著负相关关系(P<0.001)。结论认为牧草中非结构性碳水化合物与蛋白质比例决定了体外发酵产气的特性。An experiment was conducted to evaluate the in vitro gas production characteristics of ten varieties of forage, using an improved in vitro gas production technique. The ten forage varieties studied were: Pennisetum sp., Secale cereale, Pennisetum purpureum, Lolium perenne, Ponnisetum hydridum, Hemarthria compressa, Sorghum sudanense, Trifolium pratense, Medicago sativa and Rumex K-1. The modeling Gompertz in vitro fermentation kinetic parameters: maximum theoretical gas production, gas production rate constant and lag time were obtained by using nonlinear regressive procedures. The significant differences between forage species, and coefficients between kinetic parameters and different chemical compositions are analyzed and discussed in this paper. The results showed that there were significant differences (P<0.05) in the in vitro gas production kinetic parameters between the different forage species analyzed. The highest (P<0.05) values of the theoretical maximum gas production, the rate constants of gas production and the lag time occurred in S. sudanense, L. perenne and P. purpureum respectively, with the lowest values of theoretical maximum gas production, rate constant of gas production and lag time were noted for R. patientia and S. sudanense respectively. Correlation analyses indicated that the theoretical maximum gas production and the cumulative gas production were both positively (P<0.001) related to the organic matter content,the NDS∶CP ratio, and the NDS-Ash∶ CP ratio, both had a negative correlation (P<0.01) to the crude protein content. The coefficients were non-significant between the rate constants of gas production and chemical compositions. However, the lag time of gas production was positively (P<0.01) related to the hemicellulose content, and negatively (P< 0.001) correlated to the NDS : CP ratio, and NDS-Ash∶ CP ratio. It is concluded that the in vitro gas production characteristics depend upon the balance of non-structural carbohydrate and crude protein.

关 键 词：体外发酵 产气量 牧草 

分 类 号：S816.6[农业科学—饲料科学] S540.99[农业科学—畜牧学]