糖基化终产物AGEs对U937巨噬细胞高密度脂蛋白受体(SR-BI)蛋白表达的影响(英文)  

Effects of advanced glycation end products on expression of scavenger receptor class B type I (SR-BI) in U937 macrophages

作　　者：彭扬[1] 张锦[1] 李莉[1] 孟馨[1] 甘宇[1] 周一军[1] 王涤非[1] 侯率[1] 

机构地区：[1]中国医科大学附属第一医院内分泌科,辽宁沈阳110001

出　　处：《中国现代医学杂志》2005年第8期1136-1140,共5页China Journal of Modern Medicine

摘　　要：目的研究诱导分化及糖基化终产物对人单核/巨噬细胞(U937细胞)高密度脂蛋白受体(SR-BI)蛋白表达的影响。方法U937细胞经PMA诱导分化,并将不同浓度或同一浓度AGEs与诱导分化48h后的U937细胞共同孵育,用免疫细胞化学法和Western印迹法检测SR-BI蛋白的表达。结果诱导分化后U937细胞SR-BI表达在24、48h逐渐升高,72h下降;100、200和400mg/LAGEs刺激后细胞表面SR-BI蛋白表达量分别是BSA组的1.44、2.38和2.77倍(P<0.05);400mg/L的AGEs作用6、12、24、48h后,U937巨噬细胞SR-BI蛋白表达量分别为0h的1.38、2.49、3.76和4.25倍(P<0.05)。结论诱导分化24及48hSR-BI蛋白表达逐渐增加,而分化72h蛋白表达下降;AGEs可增加U937巨噬细胞SR-BI蛋白表达且呈浓度和时间依赖性。[Objective]To learn the effects of differentiation and AGEs on expression of SR-BI in U937 macrophages. U937 cells were cultured with 100 nM PMA in order to differentiate to macrophages. U937 macrophages were cultured with AGEs at different concentration for 24 hours and at a concentration of 400 mg/L for different time. A immunocytochemical method was used to detect SR-BI protein during the PMA-induced differentiation. Western blotting analysis was used to measure SR-BI protein expression stimulated by AGEs. Immunocytochemistry showed that after exposure of U937 cells to PMA for 24, 48, 72 hours, the average integrated optical density values of SR-BI protein expression in U937 cells were (15.94±3.56), (27.86±4.39) and (9.08±2.37), with the first two groups higher than that in 0 h group (7.76±1.74, P <0.05). Western Blot showed that exposure of U937 macrophages to AGEs at different concentrations (100, 200, 400 mg/L) for 24 hours resulted in a 1.44-fold, 2.38-fold and 2.77-fold increase in the expression of SR-BI protein in U937 macrophages comparing with BSA control group (P <0.05). Meanwhile, exposure of U937 macrophages to AGEs at the concentration of 400 mg/L for 6, 12, 24, 48 hours resulted in 1.38-fold, 2.49-fold, 3.76-fold and 4.25-fold increase in the expression of SR-BI protein in U937 macrophages comparing with 0 h group(P<0.05). [Conclusions] The data suggest that PMA-induced differentiation can increase the expression of SR-BI protein in U937 macrophages at 24, 48 hours, and decrease at 72 hours. U937 macrophages that were incubated in medium containing AGEs showed an increase in the expression of SR-BI protein in a time and dose dependent manner.

关 键 词：糖基化终产物 巨噬细胞 高密度脂蛋白受体 清道夫受体BI 动脉粥样硬化 

分 类 号：R363[医药卫生—病理学]