氯氧甲苯咪唑诱导白血病细胞凋亡的实验研究  

Experimental Study on Apoptosis in Leukemia Cells Induced by Econazole

作　　者：刘芳[1] 邹萍[1] 张敏[1] 吴耀辉[1] 肖娟[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院,血液病研究所,武汉430022

出　　处：《中国实验血液学杂志》2005年第3期391-393,共3页Journal of Experimental Hematology

基　　金：国家自然科学基金资助项目(编号30370595)

摘　　要：为了研究氯氧甲苯咪唑(econazole)诱导鼠粒单核白血病细胞WEHI3凋亡的机制,利用AnnexinⅤ/PI双染实验测定细胞凋亡,Fura2荧光负荷技术检测细胞内游离钙离子浓度([Ca2+]i),并抽取WEHI3细胞内质网部分蛋白,利用Westernblot测定caspase7、caspase12的蛋白表达。结果显示:econazole作用后WEHI3出现典型的凋亡改变,细胞内游离[Ca2+]i较对照明显升高,caspase12和caspase7表达随着细胞内钙离子浓度的提高而逐渐增加。结论:econazole能诱导WEHI3细胞凋亡,caspase12在此过程中起重要作用。To investigate apoptosis of mouse leukemia cell (WEHI-3) induced by econazole and its mechanism, apoptosis induced by econazole was examined by flow cytometry, while free calcium ([Ca 2+]i) was determined by Fura-2 fluorescein load techinique. The protein was isolated from endoplasmic reticulum of WEHI-3 cells, and then the expression of caspase-12 and caspase-7 was evaluated by Western blot. The results showed that WEHI-3 exhibited typical change of apoptosis when it was treated by econazole, [Ca 2+]i was significantly higher in comparison with the control. The expression of caspase-12 and caspase-7 enhanced as the econazole concentration incrcased. In conclusion, econazole can induce WEHI-3 cell apoptosis and the caspase-12 plays a key role in this process.

关 键 词：氯氧甲苯咪唑 细胞凋亡 细胞质[Ca^2+]i CASPASE-12 

分 类 号：R733.7[医药卫生—肿瘤] R979.1[医药卫生—临床医学]