外周血γδT细胞在胰腺癌裸鼠过继免疫治疗中的作用  

Anti-pancreatic cancer effects of human peripheral γδT cells in a mouse tumor model

作　　者：戴梦华[1] 何维[2] 赵玉沛[1] 许春萍[2] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院基本外科,100730 [2]中国医学科学院基础医学研究所免疫教研室

出　　处：《中华外科杂志》2005年第11期726-729,共4页Chinese Journal of Surgery

摘　　要：目的探讨外周血γδT细胞在胰腺癌裸鼠种植瘤体内过继免疫治疗中的作用。方法用胰腺癌细胞株Cap1注入BALB/c裸鼠皮下建立胰腺癌裸鼠种植瘤模型。裸鼠随机分为3组,每组10只。A组为γδT细胞治疗,B组为αβT细胞治疗,C组给以无血清RPMI1640作为对照。从胰腺癌患者外周血中提取淋巴细胞,体外特异性扩增γδT细胞后注入裸鼠腹腔内,观察种植瘤裸鼠生存时间、肿瘤体积和肿瘤病灶坏死程度。结果A组成瘤率9/10,B、C组均为8/10(P>0.05)。当肿瘤生长到16、37和52d时,A组与B、C组肿瘤体积差别具有统计学意义(P值分别为0.019和0.022)。120d时C组8只种植瘤裸鼠全部死亡,B组有5只死亡,A组有3只死亡,3组之间死亡率差异具有统计学意义(P=0.023);KruskalWallis检验生存时间存在统计学差异(P=0.036)。γδT细胞治疗组中有4只肿瘤病灶出现坏死脱落,B和C组中各有1只肿瘤出现坏死;肿瘤生长到1个月后,B组和C组中裸鼠发生头部、眼眶、尾部、会阴部和对侧腹股沟区转移,A组仅1例发生对侧腹股沟部转移;A组中有1例裸鼠种植瘤脱落消失,而B组和C组均无肿瘤脱落、坏死消失发生。结论外周血γδT细胞对胰腺癌裸鼠种植瘤具有显著的抑瘤作用。Objective To explore the adoptive immunotherapy effect of peripheral γδT cells in pancreatic cancer nude mice model. Methods Thirty BALB/c nude mice were inoculated subcutaneously 5×105 Cap-1 cells to regularly develope hypodermal tumors, and then divided into 3 groups randomly, γδT cells, αβT cells and control group. 2.5×106 γδT cells or αβT cells or 100 μl RPMI-1640 were respectively injected into abdominal cavity of mice, combined with 104 U rhIL-2 for 3 times. Tumor volume, the survival rate and anti-carcinogenic rate of three groups were compared. Results Eight control nude mice developed hypodermal tumors,which progressively increased in size, and animals had a mean survival of 88 d. Nine nude mice in γδT cells group and eight in αβT cells group developed tumors (P>0.05). Tumor growth was arrested and tumor size was reduced remarkably in γδT cells group. Mean survival was increased to 113 d with less rate of tumor metastasis and more cases of tumor necrosis in γδT cells group when compared with αβT cells group and controls. Conclusions The anti-tumor effects of γδT cells against pancreatic cancer are better than those of αβT cells and control groups,and might be promising in the adoptive immunotherapy of pancreatic cancer.

关 键 词：γδT细胞 过继免疫治疗 外周血 Kruskal-Wallis检验 BALB/C裸鼠 胰腺癌细胞株 细胞治疗 生存时间 肿瘤病灶 肿瘤体积 肿瘤生长 种植瘤 胰腺癌患者 统计学差异 淋巴细胞 腹股沟区 腹股沟部 抑瘤作用 C组 B组 坏死 瘤体内 

分 类 号：R735.9[医药卫生—肿瘤]