高血糖大鼠血脑屏障的超微形态及免疫组化研究  

The ultrastrucural and immunohistochemical study of BBB in streptozocin induced hyperglycemic rats

作　　者：郭徽[1] 张胜兰[1] 姜兆顺[1] 蒲传强[2] 郝延磊[2] 张凤英[2] 

机构地区：[1]济南军区总医院内分泌科,济南250031 [2]解放军总医院神经内科

出　　处：《解放军医学杂志》2005年第6期517-519,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家自然科学基金(编号39670267);军队医药卫生基金(编号96M123)资助课题

摘　　要：目的探讨糖尿病的慢性高血糖对血脑屏障(BBB)超微形态及其内皮细胞ICAM-1表达的影响.方法采用SD大鼠建立高血糖模型.以透射电镜和免疫组化方法动态观察3天～8个月不同时程高血糖条件下,BBB超微结构的改变及其内皮细胞ICAM-1的表达.结果高血糖大鼠3天～2个月时,BBB改变不明显,有少量ICAM-1表达;3个月时出现明显的BBB改变,ICAM-1大量表达;6～8个月时改变继续加重,ICAM-1仍保持明显表达,范围更广.对照组BBB无改变及内皮细胞ICAM-1无表达.结论长期高血糖能导致大鼠BBB形态异常和内皮细胞ICAM-1表达增加.后者可能是高血糖导致脑组织毛细血管结构及功能异常的重要病理学基础.Objective To investigate the hyperglycemic effect on ultrastructural morphology and intercellular adhension molecule 1(ICAM 1) expression of BBB. Methods Hyperglycemia model was established by intravenous injection of streptozocin in SD rats. A dynamic observation on the changes of the ultrastructure and the ICAM 1 expression on endothelium of BBB in SD rats with hyperglycemia for 3 days to 8 months was carried out by transmission electron microscopy and immunohistochemical method. Results Swelling was slight in endothelium of BBB of rats with hyperglycemia for 3 days, became obvious and occurred in endfoot of astrocyte for 1 to 2 months, and some microthrombi appeared in cavity of capillary with various malformation and apoptosis was found in endothelium of BBB and neuron surrounding abnormal capillary for 3 to 8 months. With immunohistochemical study, ICAM 1 was expressed sparsely on endothelium of BBB in 3 days after injection of streptozocin, became markedly strong in 3 months, and progressed further in 6 to 8 months. There was no changes on BBB in both SD rats and negative controls. Conclusion Long term hyperglycemia could cause obvious ultrastructural changes of BBB, associated with ICAM 1 expression on endothelium of BBB induced by hyperglycemia

关 键 词：高血糖大鼠 超微形态 免疫组化研究 ICAM-1表达 血脑屏障(BBB) 内皮细胞 免疫组化方法 慢性高血糖 高血糖模型 病理学基础 SD大鼠 动态观察 透射电镜 超微结构 形态异常 功能异常 血管结构 糖尿病 对照组 脑组织 

分 类 号：R151.41[医药卫生—营养与食品卫生学] R730.261[医药卫生—公共卫生与预防医学]