血管瘤和血管畸形组织中糖皮质激素受体及其亚型的表达及意义  

Glucocorticoid receptor isoforms in hemangioma and vascular malformation

作　　者：王广欢[1] 蒋学武[1] 李建宏[1] 陈中献[1] 贺飞[1] 

机构地区：[1]汕头大学医学院第二附属医院小儿外科,汕头515041

出　　处：《中华小儿外科杂志》2005年第6期301-304,共4页Chinese Journal of Pediatric Surgery

摘　　要：目的测定血管瘤和血管畸形组织中糖皮质激素受体(GR)及其α受体亚型(GRα)的表达情况,了解它们与血管瘤增生、消退的关系并进而探讨糖皮质激素治疗血管瘤和血管畸形的机制。方法采用免疫组织化学方法检测经病理证实的33例增生期、32例消退期及7例血管畸形标本组织中糖皮质激素受体和糖皮质激素受体α亚型的表达情况。结果GR在血管瘤和血管畸形组织标本中的阳性灰度值分别为(126.95±6.41)、(121.43±6.58),差别无统计学意义。但与正常皮肤的测得值(108.63±6.80)比较,两者均明显增高(P<0.01)。GRα在血管瘤、血管畸形和正常皮肤组织标本中的阳性灰度值分别为(127.32±5.36)、(120.43±6.13)和(109.88±5.28),差别均有统计学意义。GR在增生期、消退期血管瘤组织中的表达分别为(127.91±4.81)、(125.97±5.78),没有统计学差别。GRα的表达分别为(127.55±6.41)、(122.47±6.25),差别均有显著的统计学意义。结论血管瘤和血管畸形组织中均有高表达的GR和GRα,GRα的表达与血管瘤的分期关系密切。GR及GRα的检测对了解血管瘤的增生、消退情况以及进一步地指导糖皮质激素治疗血管瘤和血管畸形有积极意义。Objective To assess the relationship between glucocorticoid receptor (GR) and its α Isoforms (GRα) and hemangioma(H) and vascular malformation(VM). Methods sections of 33 proliferative hemangioms, 32 involuting hemangiomas and 7 vascular malformations were immunostained for GR and GRα. Results The GR-positive gray scale in hemangioms, vascular malformations, normal skin were ((126.95)±(6.41))、((121.43)±(6.58)) and ((108.63)±(6.80)) respectively. There was no difference between hemangiomas and vascular malformations. But the difference between hemangioms (P<(0.01)), vascular malformations (P<(0.01)) and normal skin was significant. The GRα-positive gray scale in hemangiomas, vascular malformations, normal skin were ((127.32)±(5.36))、((120.43)±(6.13)) and ((109.88)±(5.28)) respectively. There was a significant difference between one another. The GR-positive gray scales were ((127.91)±(4.81))、((125.97)±(5.78)), and GRα’s were ((127.55)±(6.41))、((122.47)±(6.25)) in proliferative involuting hemangiomas respectively. There was no difference in GR. The difference in GRαexpression between proliferative and involuting hemangiomas was significant. Conclusions GR and GRαare expressed in hemangioma and vascular malformation. The expression of GRαis closely related to the stage of hemangiomas. GR and GRαexpressions provide a guidance for steroid management in hemangioma and vascular malformation.

关 键 词：血管畸形 糖皮质激素治疗 表达及 免疫组织化学方法 糖皮质激素受体Α 皮肤组织标本 表达情况 GRΑ α受体亚型 血管瘤组织 统计学 病理证实 正常皮肤 消退情况 增生期 消退期 灰度值 差别 高表达 检测 阳性 

分 类 号：R732.2[医药卫生—肿瘤] R135.1[医药卫生—临床医学]