脉络膜血管铺片技术在氪激光诱发大鼠脉络膜新生血管定量分析中的应用  

Quantitative Analysis of Rat Choroidal Neovascularization Induced by Krypton Laser Photocoagulation Using Stretched Preparation of Choroidal Neovascularization

作　　者：陈学国[1] 何守志[1] 赵军[1] 史雪辉[1] 

机构地区：[1]解放军总医院眼科,北京市100853

出　　处：《中国激光医学杂志》2005年第3期146-149,共4页Chinese Journal of Laser Medicine & Surgery

基　　金：全军十五课题(02M014)。

摘　　要：目的探讨脉络膜血管铺片技术在氪激光诱发挪威棕色(BN)大鼠视网膜脉络膜新生血管(CNV)定量分析中的应用价值。方法成年挪威棕色大鼠20只,分成4组,每组5只,分别于氪激光光凝后第7、14、21和28天用高分子量的异硫氰酸荧光素葡聚糖对脉络膜血管进行灌注。灌注固定后,祛除眼前节,小心揭除神经视网膜层,获得色素上皮层-脉络膜-巩膜复合体标本。荧光显微镜观察CNV形态并照相。计算机图像分析软件测定CNV区域面积。结果视乳头周围的CNV与周围的脉络膜比较呈网状高荧光斑。从第7天至28天新生血管的面积逐渐增加(P<0.01)。第7、14、21和28天新生血管面积分别为(12408±2988)μm2、(28543±4422)μm2、(32823±4028)μm2和(34003±5126)μm2。第14天脉络膜新生血管面积近似7d的2倍(P<0.01),第14、21和28天脉络膜新生血管面积之间差别无显著意义(P>0.05)。结论脉络膜血管平铺是脉络膜新生血管定性定量分析的可靠方法,可用于CNV病因学、发病机制及药物治疗效果的研究。Objective To explore applied value of stretched preparation of choroidal neovascularization for quantitative analysis of rat choroidal neovascularization (CNV) induced by krypton laser photocoagulation.Methods Twenty adult Brown Norwey rats were divided into 4 groups.There were 5 rats in each group. Choroidal neovascularization was infused with high molecular weight FITC-dextran at d 7,14,21 and 28. Cornea, lens and nerve retina were removed and RPE-choroid-sclera flat mount was obtained. CNV was observed by fluorescence microscope. Area of CNV was measured by computer image analysis software.Results Peripapillary CNV induced by krypton laser photocoagulation was presented hyperfluorescence.Area of CNV gradually increased from d 7 to d 28(P<0.01). Area of CNV at d 7,14,21 and d 28 was (12408±2988)μm^2,(28543±4422)μm^2,(32823±4028)μm^2,(34003±5126)μm^2 respectively that at d 14 was about 2 times of. area of CNV at d 7 (P<0.01). No significant difference of area of CNV was seer at d 14,21 and d 28.Conclusions The stretched preparation of choroidal neovascularization is a reliable method and could use in the study of etiology, pathogenesis and therapeutic efficacy.

关 键 词：脉络膜新生血管 脉络膜血管 激光诱发 铺片技术 大鼠 异硫氰酸荧光素 定性定量分析 血管面积 神经视网膜 计算机图像 视乳头周围 CNV 应用价值 激光光凝 高分子量 灌注固定 膜复合体 微镜观察 分析软件 治疗效果 发病机制 

分 类 号：R773.4[医药卫生—眼科]