聚合酶链反应检测乳腺癌骨髓微小转移的意义  

PCR analysis of bone marrow micrometastasis in primary breast cancer

作　　者：于泽平[1] 罗开[1] 王震龙[1] 王少华[1] 王卫红[1] 

机构地区：[1]南京军区南京总医院解放军普通外科研究所,江苏南京210002

出　　处：《医学研究生学报》2005年第6期520-522,共3页Journal of Medical Postgraduates

摘　　要：目的:探讨聚合酶链反应(PCR)检测乳腺癌骨髓微小转移方法的可能性及其意义,寻找一种特异性强、灵敏度高的技术检测骨髓微转移灶。方法:采用巢式PCR,通过扩增乳腺组织特异性基因hMAMmRNA,对43例乳腺癌患者骨髓hMAMmRNA表达情况进行分析。结果:采用双侧髂前上嵴或髂后上嵴多点穿刺的方法,hMAMmRNA阳性率达55.81%(24/43)。结论:骨髓检测微转移相对于传统的组织学方法有明显进步,将对乳腺癌早期转移的诊断以及指导临床治疗和预后判断产生深刻影响。Objective: To evaluate the possibility to a nested reverse transcription-polymerase chain reaction (RT-PCR) assay to measure bone marrow micrometastasis in primary breast cancer, and to develop a highly sensitive and specific detection method to detect bone marrow micrometastasis.Methods:hMAM mRNA expression in bone marrow of 43 patients with primary breast was tested by nested RT-PCR assay.Results:Twenty-four patients of 43 patients bone marrow samples, taken from bilateral iliac crest, were positive(55.81%) for hMAM.Conclusion:The use of nested RT-PCR to detect bone marrow micrometastasis is a significant development compared with traditional histological method.

关 键 词：乳腺癌 骨髓微小转移 聚合酶链反应 

分 类 号：R737.9[医药卫生—肿瘤]