猪繁殖与呼吸综合征病毒缺失变异株的基因组特征  

Genomic Characterization of a Mutant of Porcine Reproductive and Respiratory Syndrome Virus with Deletion

作　　者：高志强[1] 郭鑫[1] 杨汉春[1] 陈艳红[1] 查振林[1] 

机构地区：[1]中国农业大学农业部预防兽医学重点开放实验室,北京100094

出　　处：《畜牧兽医学报》2005年第6期578-584,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家重点基础研究发展规划项目(G1999011901)

摘　　要：对猪繁殖与呼吸综合征病毒(PRRSV)分离株HB-2(sh)/2002的全基因组序列进行了测定与分析.该毒株基因组全长为15 373 nt(不包括PolyA尾),与国内外美洲型PRRSV分离株全序列相似性介于88.7%～95.1%之间.序列分析表明,该毒株是1个天然存在缺失的变异毒株,其ORF1a的Nsp2存在编码12个氨基酸的连续36个核苷酸的缺失,ORF3存在编码1个氨基酸的3个核苷酸的缺失.这是国内外首次发现PRRSV存在缺失变异现象,研究结果补充和丰富了PRRSV毒株的基因组信息数据,为深入研究该毒株的遗传与变异及其与生物学特性的关系奠定了基础.The full-length genome of PRRSV HB-2(sh)/2002 was sequenced and analyzed. The size of the virus genome was 15 373 nucleotides, excluding the poly(A)tail. Comparative analysis with the genomic sequences of domestic and abroad North American isolates showed that HB-2(sh)/2002 shared 88.7%~95.1% identity with them. The isolate was a mutant of PRRSV with unique deletion, namely a 36 nucleotides deletion in Nsp2 and 3 nucleotides deletion in GP3 were found. This is first finding that PRRSV exists deletion variation. Our results enrich information data of PRRSV genome and provide a valuable basis for further study of biological characteristics, heredity and mutation of PRRSV.

关 键 词：猪繁殖与呼吸综合征病毒 基因组特征 缺失 变异株 PRRSV 全基因组序列 序列相似性 生物学特性 变异毒株 首次发现 信息数据 研究结果 分离株 国内外 核苷酸 氨基酸 分析表 编码 全长 遗传 

分 类 号：S852.65[农业科学—基础兽医学] S858.32[农业科学—兽医学]