BQ123对低温诱发的肉鸡肺血管重塑的影响  

Effect of BQ123 on Pulmonary Vascular Remodeling in Low-temperature-induced Broiler Chickens

作　　者：范春艳[1] 乔健[1] 赵立红[1] 刘文菊[1] 欧德渊[1] 杨鹰[1] 高铭宇[1] 

机构地区：[1]中国农业大学动物医学院,北京100094

出　　处：《畜牧兽医学报》2005年第6期606-611,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金项目(30070567;30371063)

摘　　要：动态观察了肺小动脉中膜平滑肌的增殖及管腔面积的改变,探讨内皮素受体拮抗剂BQ123对低温诱发的肉鸡肺血管重塑的影响.200只16日龄AA肉鸡随机均分为4组: 常温(20 ℃)对照组、低温(7～9 ℃)组、低温低剂量BQ123组和低温高剂量BQ123组.23日龄、30日龄时测定肺动脉压,并取肺组织做石蜡切片, 以Weigert间苯二酚复红染色,形态学计算机图象分析法测定肺细小动脉外径和内径、管总面积和管腔面积,计算中膜厚度占外径百分值(%)mMTPA及管壁面积/管总面积WA/TA(%).结果显示:(1)BQ123抑制低温肉鸡平均肺动脉压的升高,23日龄时低温高剂量BQ123组显著低于低温组(P＜0.05),30日龄时低温低剂量BQ123组和低温高剂量BQ123组均极显著低于低温组(P＜0.01);(2)BQ123抑制低温肉鸡肺小动脉WA/TA(%)的升高,30～50 μm的肺小动脉,低温组极显著高于低温低剂量BQ123组及低温高剂量BQ123组(P＜0.01),其它分级的肺小动脉组间差异性与此类似;(3) BQ123抑制低温肉鸡肺小动脉的mMTPA的升高,30～50 μm的肺小动脉,23日龄时低温组极显著高于低温低剂量BQ123组和低温高剂量BQ123组(P＜0.01),30日龄时低温组显著高于低温低剂量BQ123组(P＜0.05)、极显著高于低温高剂量BQ123组(P＜0.01),30 μm以下的肺小动脉组间差异性与此类似,50～120 μm的差异不显著.这些结果提示,内皮素受体拮抗剂BQ123明显抑制了低温诱发的肉鸡肺动脉高压和肺血管重塑,因此推测内源性内皮素(ET)在低温诱发的肉鸡肺动脉高压和肺血管重塑中起重要作用.The effect of BQ123,a selective ETA-receptor antagonist, on pulmonary vascular structural remodeling induced by low ambient temperature was investigated through observing the dynamic change of pulmonary arterioles medial thickness and pulmonary blood vessel endometry area caused by proliferous smooth muscle cells in broiler chickens. Two hundred 16-day old commercial broiler chickens were randomly divided into 4 groups as follows: 50 birds were exposed to normal temperature(20 ℃) and injected normal saline to abdominal cavity as control group, 50 birds were exposed to low temperature (7~9 ℃) and injected normal saline to abdominal cavity as low temperature group,50 birds were exposed to low temperature and injected low dose BQ123 to abdominal cavity as low dose BQ123 group,and 50 birds were exposed to low temperature and injected high dose BQ123 to abdominal cavity as high dose BQ123 group. Pulmonary arterial pressure (PAP ) was determined as index of pulmonary hypertension. The measurement of ratio of vessel wall area to vessel total area (WA/TA) and mean medial thickness in pulmonary arterioles (mMTPA) were accomplished by using the microscopic image analysis program. The results showed as follows,(1)BQ123 significantly inhibited the increase of mPAP(kPa): high dose BQ123 group was lower than low temperature group at 23 days(P<0.05);high dose BQ123 group and low dose BQ123 group were obviously lower than low temperature group at 30 days(P<0.01);(2)BQ123 inhibited the increase of WA/TA of pulmonary arterioles: the value of 30~50 μm pulmonary arterioles of low temperature group was obviously higher than that of low dose BQ123 group and high dose BQ123 group (P<0.01); It bears an analogy to the differences between groups in the other ranks of pulmonary arterioles;(3)BQ123 made mMTPA of pulmonary arterioles to be reduced: to 30~50 μm pulmonary arterioles,at the age of 23, low temperature group was obviously higher than low dose BQ123 group and high dose BQ123 group(P<0.01); at the age of 30, low temperatue

关 键 词：血管重塑 低温 诱发 内皮素受体拮抗剂 肺小动脉 肺动脉高压 30日龄 肺动脉压 高剂量 低剂量 肺细小动脉 计算机图象 3日龄 动态观察 AA肉鸡 石蜡切片 间苯二酚 差异性 面积 抑制 平滑肌 20℃ 肺组织 分析法 形态学 膜厚度 

分 类 号：S858.312.3[农业科学—临床兽医学] Q463[农业科学—兽医学]