猪LPL基因内含子3的克隆、测序及多态性研究  

Cloning,Sequencing and Polymorphism of Intron 3 of Porcine LPL

作　　者：张振波[1] 雷明刚[1] 邓昌彦[1] 熊远著[1] 

机构地区：[1]华中农业大学农业部猪遗传育种重点开放实验室,武汉430070

出　　处：《畜牧兽医学报》2005年第6期627-630,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家"973"(G2000016105);"863"(2001AA243031);博士点基金(20020504006)

摘　　要：Lipoprotein lipase (LPL) gene is a multifunctional protein, playing a major role in the hydrolysis of triglycerides in chylomicrons and very low density lipoproteins (VLDL). According to cDNA of Landrace breed (GenBank accession: X62984), a pair of primers was designed for amplification of intron 3. Sequencing analysis indicated that a G1200A exits in Large White breed by cloning and sequencing. The mutation can be detected by PCR-EcoT22I-RFLP. Polymorphism analysis in a resource family showed that significant difference exits in carcass traits. Pigs with AA genotype had more 6th-7th rib fat thickness (13%,P< 0.05), thorax-waist-fat thickness (11.7%,P=0.065 3), buttock fat thickness (13.1%,P=0.073 0) and average fat thickness (10.4%,P=0.050 3) than pigs with BB genotype. LPL gene showed mainly in the pattern of additive effect and all the dominant effect were not significant. The value of additive effect of 6th-7th rib fat thickness, buttock fat thickness and average fat thickness was -0.17±0.07(P< 0.05),-0.12±0.06( P< 0.05),-0.12±0.06(P< 0.05),respectively. The allelic frequency is significantly different between indigenous Chinese breeds and European breeds.Lipoprotein lipase (LPL) gene is a multifunctional protein, playing a major role in the hydrolysis of triglycerides in chylomicrons and very low density lipoproteins (VLDL). According to cDNA of Landrace breed (GenBank accession: X62984), a pair of primers was designed for amplification of intron 3. Sequencing analysis indicated that a G1200A exits in Large White breed by cloning and sequencing. The mutation can be detected by PCR-EcoT22I-RFLP. Polymorphism analysis in a resource family showed that significant difference exits in carcass traits. Pigs with AA genotype had more 6th-7th rib fat thickness (13%,P< 0.05), thorax-waist-fat thickness (11.7%,P=0.065 3), buttock fat thickness (13.1%,P=0.073 0) and average fat thickness (10.4%,P=0.050 3) than pigs with BB genotype. LPL gene showed mainly in the pattern of additive effect and all the dominant effect were not significant. The value of additive effect of 6th-7th rib fat thickness, buttock fat thickness and average fat thickness was -0.17±0.07(P< 0.05),-0.12±0.06( P< 0.05),-0.12±0.06(P< 0.05),respectively. The allelic frequency is significantly different between indigenous Chinese breeds and European breeds.

关 键 词：多态性研究 内含子 L基因 极低密度脂蛋白 克隆 测序 三酰甘油 脂蛋白脂酶 LPL 脂肪组织 代谢障碍 水解酶 猪 脂肪酸 

分 类 号：S852.23[农业科学—基础兽医学] Q753[农业科学—兽医学]