乏氧对人舌癌Tca8113细胞尿激酶型纤溶酶原激活物表达的影响  

Effect of Hypoxia on the Expression of Urokinase-type Plasminogen Activator in Human Tongue Squamous Cell Carcinoma Cells (Tca8113)

作　　者：王升志[1] 梁新华[2] 成洲[1] 毕洪广[1] 毛祖彝[2] 王大章[2] 

机构地区：[1]青岛大学医学院附属烟台毓璜顶医院口腔颌面外科,山东烟台264000 [2]口腔生物医学工程教育部重点实验室,四川大学四川成都610041

出　　处：《华西口腔医学杂志》2005年第3期211-213,共3页West China Journal of Stomatology

基　　金：山东省优秀中青年科学家科研奖励基金资助项目(03BS038);广东省医学科研课题资助项目(A2002434)

摘　　要：目的研究乏氧对人舌癌Tca8113细胞尿激酶型纤溶酶原激活物(uPA)表达的影响,探讨乏氧与口腔鳞癌侵袭、转移的关系。方法采用免疫组化染色和流式细胞术,对Tca8113细胞在不同乏氧时间(0、3、6、12、24h)下的uPA蛋白表达进行染色和定量分析。结果乏氧可促进人舌癌Tca8113细胞uPA蛋白表达,而且随着乏氧时间3h→6h→12h→24h的延长,uPA表达逐渐增高。结论乏氧可通过激活肿瘤细胞高表达uPA而促进口腔鳞癌侵袭和转移。Objective To observe the expression of urokinase-type plasminogen activator (uPA) in human tongue squamous carcinoma cells (Tca8113) under hypoxia, in order to explore the relation between hypoxia and invasion and metastasis of oral cancer.Methods Under different hypoxic times(0,3,6,12,24 h), the expression of uPA protein was examined quantitatively using immunohistochemical technique (IH) and flow cytometry (FCM).Results Hypoxia promoted the expression of uPA. The longer hypoxic time was, the higher expression of uPA was.Conclusion Hypoxia can promote invasion and metastasis of oral squamous carcinoma through stimulating the expression of uPA.

关 键 词：乏氧 尿激酶型纤溶酶原激活物 舌鳞状细胞癌 侵袭 转移 

分 类 号：R739.8[医药卫生—肿瘤]