血清预培养促进神经干细胞的增殖  

Pro-culture with serum promoting neural stem cell proliferation

作　　者：历俊华[1] 郑淑荣[1] 万虹[1] 张绍东[1] 

机构地区：[1]北京市神经外科研究所,北京市100050

出　　处：《中国康复理论与实践》2005年第5期339-340,共2页Chinese Journal of Rehabilitation Theory and Practice

基　　金：国家自然科学基金(No.30370543)

摘　　要：目的介绍一种时间短、花费少的神经干细胞培养方法。方法先用含10%胎牛血清的DMEM培养液预培养神经干细胞48 h,换含表皮生长因子、碱性成纤维细胞生长因子、B27的DMEM/F12培养液继续培养干细胞球。血清诱导分化后行nestin免疫荧光染色。结果血清预培养48 h后即可见神经干细胞聚球,nestin免疫荧光染色为阳性。结论血清预培养可使神经干细胞聚球速度加快,促进神经干细胞增殖。ObjectiveTo introduce a method of promoting neural stem cells proliferation.MethodsNeural stem cells were cultured with medium DMEM containing 10% fatal bovine serum for 48 h, then changed medium DMEM/F12 containing epidermal growth factor, basic fibroblast growth factor and B27. Immunohistochemistry was used to identify nestin expression.ResultsNeural stem cells got together fast and formed neurospheres within 48 h and expressed nestin. ConclusionPro-culture with serum can promotes neural stem cell proliferation.

关 键 词：神经干细胞 预培养 血清 增殖 

分 类 号：R741.05[医药卫生—神经病学与精神病学]