地塞米松对实验性自身免疫性脑脊髓炎大鼠MCP-1表达的影响  

Effect of dexamethesone on monocyte chemoattractant protein 1 mRNA expression in Wistar rat with experimental autoimmune encephalomyelitis

作　　者：刘华[1] 刘颖[1] 梁丽云[1] 马存根[1] 

机构地区：[1]大同医学专科学校脑科学研究所,037008

出　　处：《山西医药杂志》2005年第6期453-455,共3页Shanxi Medical Journal

基　　金：山西省2003年度回国留学人员科研基金资助项目(200369)

摘　　要：目的为探讨地塞米松(DXM)对实验性自身免疫性脑脊髓炎(EAE)大鼠临床指标和单核细胞趋化蛋白1(MCP1)mRNA的影响。方法将正常大鼠、完全抗原和百日咳毒素原液免疫后的大鼠免疫后7d,分成:正常大鼠、EAE组和DXM组。DXM组给予DXM腹腔注射,另外两组给予生理盐水注射。取脑和脊髓制成石蜡切片,进行苏木素伊红(HE)染色和MCP1mRNA的原位杂交(ISH),并比较各项临床指标。结果DXM组的发病率、临床评分和MCP1mRNA的阳性细胞数显著性降低(P<0.01)。结论进一步证实DXM的使用可对EAE有治疗作用。Objective To study the immunosuppressive effect of injection of dexamethasone(DXM) on monocyte chemoattractant protein 1 (MCP 1) mRNA expression in experimental autoimmune encephalomyelitis (EAE) Wistar rats.Methods The normal Wistar rats,guinea pig spinal cord homogenate,complete Freund′s adjuvant (CFA) and pertussis toxin (PT) immunized Wistar rats were divided into three groups:the normal Wistar rats,group EAE,group DXM.The brains and spinal cords were removed for hematoxylin eosin (HE) staining and in situ hybridization (ISH) staining.Results DXM group made a striking decrease in morbidity,clinical score and MCP 1 mRNA positive cell (P<0 05).Conclusion DXM has immunosuppressive effect on EAE,ie,a curative effect on it.

关 键 词：实验性自身免疫性脑脊髓炎 MCP-1 地塞米松 单核细胞趋化蛋白 生理盐水注射 临床指标 正常大鼠 mRNA 百日咳毒素 DXM 完全抗原 腹腔注射 石蜡切片 原位杂交 临床评分 治疗作用 免疫后 EAE 苏木素 发病率 显著性 细胞数 

分 类 号：R744[医药卫生—神经病学与精神病学]