胃癌及非癌细胞DNA流式细胞光度分析  

Flew Cytometric Analysis of Cancer and Precancerous Lesions of Stomach

作　　者：李勇[1] 丛庆文[1] 马志学[1] 吴祥德[1] 焦秉忠 吴国祥[1] 于凤玲[1] 左连富[2] 郭建文[2] 

机构地区：[1]河北医学院第四医院,石家庄市050011 [2]河北省肿瘤研究所

出　　处：《中国肿瘤临床》1994年第2期116-119,共4页Chinese Journal of Clinical Oncology

摘　　要：用流式细胞光度术对127例胃组织样品进行DNA含量分析.光镜下分为正常粘膜,浅表性胃炎,慢性萎缩性胃炎,轻、中、重度不典型增生和胃癌共7组.以DNA指数表示细胞核相对DNA含量.结果:重度不典型增生及胃癌组均与前5组有极显著差异,对其中64例的细胞周期分析表明:正常组与浅表性胃炎组之间S期细胞比率、细胞增殖指数无显著差异,但与其他组有显著差异,胃癌组细胞增殖指数与除重度不典型增生之外的其他组有显著差异.因此,DNA含量异常,S期细胞比率及细胞增殖指数明显增高,为胃粘膜潜在恶性或恶变的客观指标.DNA flow cytometric analysis was performed on 127 human gastric tissue samples. Histologi-cally,the samples were taken from cases consisting of 7 kinds of situations i. e. , normal gastric mucosa, superficial gastritis,chronic atrophic gastritis,mild dysplasia,moderate dysplasia,severe dysplasia and cancer. DNA Index(DI) was used to represent the relative DNA content of nuclei. The DI of severe dysplasia or cancer was significantly higher than that of the other 5 groups,and .there was no significant difference among the latter 5 groups. In addition, cell cycle analysis of the 64 samples was carried out. The results showed that the percentage of the S phase cell and proliferiting index of normal gastric mucosa were not significantly different from superficial gastritis,but were different from the other groups. Proliferating index of gastric cancers was significantly higher than that in the other groups except severe dysplasia group. All the results showed that the abnormality of DNA content,remarkabl ncreased percentage of S phase cells and proliferating index might be construed as signs indicating the potential or virtually malignant change of the gastric mucosa.

关 键 词：癌前病变 流式细胞 DNA 胃肿瘤 

分 类 号：R735.203[医药卫生—肿瘤]