生存素在姜黄素诱导胃癌SGC-7901细胞凋亡中的表达及其意义  

Expression and Function of Survivin in the Apoptosis of Gastric Cancer Induced by Curcumin

作　　者：谭小平[1] 徐三平[1] 郭锐[1] 谢平[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院消化内科,武汉430022

出　　处：《临床消化病杂志》2005年第3期119-121,共3页Chinese Journal of Clinical Gastroenterology

摘　　要：目的生存素是近年发现的作用最强的细胞凋亡抑制因子,通过研究姜黄素诱导胃癌细胞凋亡过程前后的生存素表达情况,探讨其用作机制。方法不同浓度的姜黄素作用于SGC-7901细胞,MTT检测细胞生长抑制率,流式细胞术检测细胞凋亡,West-ernblot分析姜黄素作用前后胃癌细胞中Survivin的表达。结果姜黄素能显著抑制体外培养的SGC-7901细胞的生长并呈量效和时效关系。流式细胞术检测到亚二倍体凋亡峰,细胞凋亡率增加。Westernblot结果提示经姜黄素作用后,Survivin表达率下降。结论姜黄素能抑制胃癌细胞SGC-7901的生长并促进其凋亡,其发生与Survivin有关。Objective Survivin is a recently discovered inhabitor of apoptosis , and it is the mostly strong inhabitor of apoptosis . This study was aimed to investigate the expression of survivin in the apoptosis of gastric cancer cell induced by curcumin. Methods SGC-7901 cells were treated with various concentrations of curcumin and the growth inhibition rates of it were accessed by WTT method. Apoptosis of gastric cancer cells were inspected by flow cytometry. The expression of survivin in gastric cancer cells were evaluated by western blot. Results Curcumin could effectively inhibit the growth of the gastric cancer cells in dose-dependent and time dependent manners, the sub-peak appeared and the apoptotic rate was increased. The expressions of survivin was decreased. Conclusion Curcumin could significantly inhibit the growth of gastric cancer cells (SGC-7901) .inducing apoptosia probably through down-regulating survivin.

关 键 词：生存素 姜黄素 胃癌细胞 凋亡 

分 类 号：R735.2[医药卫生—肿瘤]