IgM抗体捕捉－生物素亲合素－ELISA检测Ⅳ型登革病毒IgM抗体及其临床意义  

MAC BA ELISA THE DETECTION OF IGM ANTIBODIES TO DENGUE VIRUS TYPE IV AND ITS CLINICAL SIGNIFICANCE

作　　者：王飞[1] 柯伟民[1] 卢建溪[1] 郭日波[1] 李刚[1] 姚集鲁[1] 罗惠蓉 廖育煌 

机构地区：[1]广州中山医科大学,广州市卫生防疫站

出　　处：《中华传染病杂志》1994年第2期78-81,共4页Chinese Journal of Infectious Diseases

摘　　要：用蔗糖丙酮抽提的鼠脑Ⅳ型登革病毒抗原和金黄色萄萄球菌诱发免疫小鼠制备Ⅳ型登革病毒腹水折体建立ＩｇＭ折体捕捉－生物素亲和素－酶联免疫吸附试验（ＭＡＣ－ＢＡ－ＥＬＩＳＡ）检测５５份Ⅳ型登革热患者血清的ＩｇＭ抗体。急性期阳性率为４２．４％（１４／３３），恢复期阳性率为９５．５％（２１／２２）。在一些Ⅳ型登革热患者血清中，ＩｇＭ抗体的出现可早至第２或３病日。本试验具有较高的敏感性、特异性、快速和仅需单份血清等优点。Immunoglobulin M antibody capture-biotin avidin-enzyme linked immunosorbentassay(MAC-BA-ELISA)was established for the detection of IgM antibodies among 55sera from patients with Dengue fever type Ⅳ with sucrose-acetone-extracted mouse brainDengue virus type Ⅳ antigen and aureus staphy-lococcus induced ascites antibodies toDengue virus type Ⅳ produced by immunized mouse.Positivity is 42.4%(14/33)inacute phase sera and 95.5%(21/22)in convalescent phase sera. The IgM antibody inthe sera appears as early as on the 2nd or 3rd days after onset of the disease from somepatients with Dengue fever type Ⅳ. This test has the advantages of relative specificity,sensitivity, rapidity and only single serais required.

关 键 词：免疫球蛋白 登革热病毒 

分 类 号：R373.33[医药卫生—病原生物学]