胰高血糖素样肽-2对短肠大鼠残留小肠吸收功能基因表达的影响  

Effects of glucagon-like peptide 2 on the absorption-related genes expressionsof residual small bowel in a rat model of short bowel syndrome

作　　者：陈吉[1] 李杭[1] 吴国豪[1] 肖虹蕾[2] 周国民[2] 

机构地区：[1]复旦大学附属中山医院普外科 [2]复旦大学医学院解剖和组胚系

出　　处：《中华消化杂志》2005年第5期270-273,共4页Chinese Journal of Digestion

摘　　要：目的研究胰高血糖素样肽-2(GLP-2)对短肠大鼠残留小肠钠葡萄糖共同转运体(SGLT1)和二肽转运体(PEPT1)的mRNA表达影响。方法将75%小肠切除大鼠分为空白对照组(空白组)、生长激素对照组(GH组)和胰高血糖素样肽2组(GLP-2组),另设一组正常进食大鼠作空白组的对照组(正常组)。术后1d起进食标准大鼠饲料。术后6d取末端回肠黏膜,用逆转录多聚酶链反应方法半定量检测其SGLT1和PEPT1的mRNA表达情况。结果残留回肠SGLT1和PEPT1的mRNA表达,空白组显著高于正常组(P<0.05),空白组和GH组及GLP2组之间差异均无统计学意义(P>0.05)。结论GLP-2术后短期应用对短肠大鼠残留回肠SGLT1和PEPT1的mRNA表达可能无显著影响。Objective To study the effect of glucagon-like peptide 2 (GLP-2) on the absorption- related genes [ sodium dependent glucose transporter 1 (SGLT1) and dipeptide transporter 1 (PEPT1)] mRNA expressions of residual small bowel in a rat model of short bowel syndrome. Methods Rats undertaken 75% small bowel resection were randomly assigned into 3 groups: short bowel control (SBC) group, growth hormone (GH) group and GLP-2 group. Another weight-matched normal rats with normal diet was used as normal (NC) group. All rats were given normal chow on the 1st postoperative day (POD). On the 6th POD, partial segment of terminal ileum was harvested to evaluate the mRNA expressions of SGLT1 and PEPT1 by semiquantified reverse transcription polymerase chain reaction. Results The mRNA expressions of both SGLT1 and PEPT1 in SBC group were significantly higher than that of NC group(P<0.05), however no significant expression difference existed among SBC, GH or GLP-2 groups(P>0.05). Conclusions A short term GLP-2 adiministration in early postoperative days may has no significant effect on mRNA expressions of SGLT1 and PEPT1 in residual ileum in rats with short bowel syndrome.

关 键 词：胰高血糖素样肽-2 短肠大鼠 小肠吸收功能 残留 基因表达 mRNA表达 逆转录多聚酶链反应 PEPT1 GLP-2 半定量检测 对照组 小肠切除 生长激素 进食标准 回肠黏膜 表达情况 短期应用 转运体 正常组 葡萄糖 术后 统计学 

分 类 号：R574[医药卫生—消化系统]