应用聚合酶链反应技术检测子宫颈组织中人乳头瘤病毒的研究  

Detection of human papillomavirus in cervicaltissues using polymerase chain reaction

作　　者：王芝芬[1] 刘丽[1] 汤华[1] 张维铭[1] 余虹[1] 李卫东[1] 

机构地区：[1]天津第二医学院附属第一中心医院,天津医学院

出　　处：《中华妇产科杂志》1994年第2期103-105,共3页Chinese Journal of Obstetrics and Gynecology

摘　　要：应用聚合酶链反应（ＰＣＲ）技术对５３例正常宫颈组织、５９例宫颈炎、１２例宫颈癌、３例非典型增生及２例尖锐湿疣的子宫颈组织进行人乳头瘤病毒（ＨＰＶ）－１６，１８的检测。结果：正常子宫颈组织中ＨＰＶ－１６，１８的检出率低于宫颈癌组织，两者差异有显著意义；宫颈癌组织中ＨＰＶ－１６，１８的检出率高于宫颈炎组织，两者差异无显著意义。提示：部分有ＨＰＶ感染的宫颈炎可导致宫颈癌。etection of human papillomavirus (HPV) 16and 18 infection by polymerase chain reaction (PCR)was carried out in patients with normal cervix 53cases, Chronic cervicitis with cervical erosion 59 cases,cervical cancer 12 cases, atypical hyperplasia 3 casesand condyioma acuminatum 2 cases. Results showedthat a significantly higher detective rate of HPV-16and 18 DNA in cervical cancer than in normal cervix,and a higher but not statistically significant positiverate than that in cervical erosion. It indicates thatsome cases of chronic cervicitis with HPV infectionmight develope into cervical carcinoma. This findingmay help in the early discovery and prevention ofcervical cancer.

关 键 词：聚合酶链反应 子宫颈 乳头状瘤病毒 

分 类 号：R737.330.4[医药卫生—肿瘤]