淋巴因子激活杀伤细胞的快速激活及其临床应用  

The rapid activation of human LAK cells andtheir inhibition on ovarian cancer at the advanced stage

作　　者：宋岩峰 赵亚南[1] 李进[1] 戚少然 朱忠勇 

机构地区：[1]福州东方医院妇产科,上海长海医院妇产科

出　　处：《中华妇产科杂志》1994年第6期332-335,共4页Chinese Journal of Obstetrics and Gynecology

摘　　要：用人脐血进行淋巴因子激活杀伤（ＬＡＫ）细胞体外快速激活，证实高浓度白细胞介素Ⅱ（ＩＬ－２）短期诱导可诱发明显ＬＡＫ活性。在一定浓度下诱导１小时，可获与常规法诱导（３～７天）类似的杀伤效应。血清学试验表明，此效应不受自体血清抑制。以短期诱导和常规诱导的两种ＬＡＫ细胞，对人卵巢癌裸鼠模型进行治疗，均获明显抑瘤作用。本研究使ＬＡＫ细胞体外诱导时间缩短，程序简化，费用降低。在此基础上，对快速ＬＡＫ细胞在晚期恶性肿瘤的临床应用作了初步探讨。he study of generation of LAK cells from cordblood following a brief exposure to interleukin-2(IL-2) demonstrated that a short time induction(15min～1h) of lymphocytes by a high concentrationof IL-2 resulted in the maturation of LAK proceusorcells and such activated cells expressed cytolytic activi-ty comparable to that of routinely induced (culturedcontinuously in IL-2 for 3～7 days) lymphocytes.Serologic test showed that this efficiency was not in-hibited by autoserum. The results of experimentaltherapy with the two kinds of LAK cells in nudemdese model of human ovarian cancer confimied thatboth types of LAK cells had remarkable inhibitory ef-fect on tumor growth. The preliminary trial in thetreatment of advanced ovarian cancer withrapid-pulsed LAK cells suggested that this methodmay serve as a new approach in the clinical applicationof tumor immunotherapy.

关 键 词：淋巴因子 杀伤细胞 卵巢肿瘤 

分 类 号：R737.310.5[医药卫生—肿瘤]