肿瘤坏死因子与干扰素协同抗卵巢癌作用的研究  

Anti-proliferative effects of tumor necrosisfactor and gamma interferon on human ova-rian cancer cell lines

作　　者：李进[1] 刘玉兰[1] 沙金燕[1] 赵亚南[1] 

机构地区：[1]第二军医大学附属长海医院妇产科

出　　处：《中华妇产科杂志》1994年第11期670-672,共3页Chinese Journal of Obstetrics and Gynecology

基　　金：国家自然科学基金

摘　　要：应用肿瘤坏死因子（ＴＮＦ）和γ干扰素（ＩＦＮγ）对靶细胞３ＡＯ和ＮＩＨ：ＯＶＣＡＲ－３卵巢癌细胞株进行抗肿瘤治疗的实验研究。结果：３ＡＯ和ＮＩＨ：ＯＶＣＡＲ－３卵巢癌细胞株对各种浓度的ＴＮＦ和ＩＦＮγ均呈现抵抗作用。ＴＮＦ和ＩＦＮγ协同用药时对靶细胞有杀伤作用，当ＴＮＦ和ＩＦＮγ的浓度均为１×１０￣４Ｕ／Ｌ时，出现对３ＡＯ和ＮＩＨ：ＯＶＣＡＲ－３卵巢癌细胞的明显杀伤作用（Ｐ＜０．０５）；当ＴＮＦ和ＩＦＮγ均为１×１０￣６Ｕ／Ｌ时，杀伤５５％的３ＡＯ和７５％的ＮＩＨ：ＯＶＣＡＲ－３卵巢癌细胞（Ｐ＜０．０１）。提示：ＩＦＮγ可增加３ＡＯ和ＮＩＨ：ＯＶＣＡＲ－３卵巢癌细胞对ＴＮＦ的敏感性，ＴＮＦ与ＩＦＮγ联合用药时有很好的协同抗卵巢癌作用。wo ovarian cancer cell lines, 3AO and NIH:OVCAR-3 , were used in this study to assess the syner-gistic effect of TNF and IFNγ in combination. The re-sult showed that these two ovarian cancer cell lineswere all resistant to TNF and IFNγ individually, butsensitive to the combined administration of TNF andIFNγ. Concentration of 1×10￣4 U/L of TNF and IFNγsignificantly inhibited 3AO and NIH : OVCAR-3 ovari-an cancer cells (P<0. 05) . Administration of 1×10￣6U/L TNF and IFNγ could inhibit the growth of 3AO cellsby 55% and NIH: OVCAR-3 cells by 75% (P<0. 01). It suggests that in some ovarian cancer cellswhich resistant to TNF could be changed to TNF sen-sitive cells by IFNγ. anticancer treatment higher anti-tumor activity could be achieved by combined adminis-tration of TNF and IFNγ.

关 键 词：肿瘤坏死因子 干扰素类 卵巢肿瘤 免疫疗法 

分 类 号：R737.310.3[医药卫生—肿瘤]